Nucleic Acids Research, Vol 24, Issue 21 4222-4226, Copyright © 1996 by Oxford University Press
J Nikawa, M Akiyoshi, S Hirata and T Fukuda
The Saccharomyces cerevisiae IRE1 gene, encoding a putative receptor- type
protein kinase, is known to be required for inositol prototrophy and for
the induction of a chaperon molecule, BiP, encoded by KAR2, under stress
conditions such as tunicamycin addition. We have characterized a yeast
gene, IRE2, which was isolated as a suppressor gene that complements the
inositol auxotrophic phenotype of the ire1 mutation. Sequencing analysis
revealed that IRE2 is identical to HAC1, which encodes a transcription
factor having a basic-leucine zipper motif. Introduction of IRE2/HAC1 into
the ire1 mutant clearly restored the expression of KAR2 upon tunicamycin
treatment. ire2/hac1-disrupted yeast cells showed not only the inositol
auxotrophic phenotype but also the tunicamycin sensitivity, and failed to
induce the expression of KAR2. These results clearly indicate that the
IRE2/HAC1 gene product plays a critical role in the induction of KAR2
expression and in the inositol prototrophy mediated by IRE1.
ARTICLES
Saccharomyces cerevisiae IRE2/HAC1 is involved in IRE1-mediated KAR2 expression
Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan. nikawa@bse.kyutech.ac.jp
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