Nucleic Acids Research, Vol 24, Issue 22 4373-4378, Copyright © 1996 by Oxford University Press
P Volkl, P Markiewicz, C Baikalov, S Fitz-Gibbon, KO Stetter and JH Miller
The hyperthermophilic archaeum, Pyrobaculum aerophilum, grows optimally at
100 degrees C with a doubling time of 180 min. It is a member of the
phylogenetically ancient Thermoproteales order, but differs significantly
from all other members by its facultatively aerobic metabolism. Due to its
simple cultivation requirements and its nearly 100% plating efficiency, it
was chosen as a model organism for studying the genome organization of
hyperthermophilic ancient archaea. By a G+C content of the DNA of 52 mol%,
sequence analysis was easily possible. At least some of the mRNA of P.
aerophilum carried poly-A tails facilitating the construction of a cDNA
library. 245 sequence tags of a poly-A primed cDNA library and 55 sequence
tags from a 1-2 kb Sau3AI- fragment containing genomic library were
analyzed and the corresponding amino acid sequences compared with protein
sequences from databases. Fourteen percent of the cDNA and >9% of
genomic DNA sequence tags revealed significant similarities to proteins in
the databases. Matches were obtained to proteins from archaeal, bacterial
and eukaryal sources. Some sequences showed greatest similarity to eukaryal
rather than to bacterial versions of proteins, other matches were found to
proteins which had previously only been found in eukaryotes.
ARTICLES
Genomic and cDNA sequence tags of the hyperthermophilic archaeon Pyrobaculum aerophilum
Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024, USA.
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