Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (256K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Ramamurthy, L
Right arrow Articles by Marzluff, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ramamurthy, L
Right arrow Articles by Marzluff, W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 24, Issue 22 4525-4534, Copyright © 1996 by Oxford University Press

ARTICLES

Increasing the distance between the snRNA promoter and the 3' box decreases the efficiency of snRNA 3'-end formation

L Ramamurthy, TC Ingledue, DR Pilch, BK Kay and WF Marzluff
Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill 27599, USA.

Chimeric genes which contained the mouse U1b snRNA promoter, portions of the histone H2a or globin coding regions and the U1b 3'-end followed by a histone 3'-end were constructed. The distance between the U1 promoter and the U1 3' box was varied between 146 and 670 nt. The chimeric genes were introduced into CHO cells by stable transfection or into Xenopus oocytes by microinjection. The efficiency of utilization of the U1 3' box, as measured by the relative amounts of transcripts that ended at the U1 3' box and the histone 3'-end, was dependent on the distance between the promoter and 3'-end box. U1 3'-ends were formed with >90% efficiency on transcripts shorter than 200 nt, with 50- 70% efficiency on transcripts of 280-400 nt and with only 10-20% efficiency on transcripts >500 nt. Essentially identical results were obtained after stable transfection of CHO cells or after injecting the genes into Xenopus oocytes. The distance between the U1 promoter and the U1 3' box must be <280 nt for efficient transcription termination at the U1 3' box, regardless of the sequence transcribed.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genes Dev.Home page
G. Chanfreau, S. A. Elela, M. Ares Jr., and C. Guthrie
Alternative 3'-end processing of U5 snRNA by RNase III
Genes & Dev., October 15, 1997; 11(20): 2741 - 2751.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. J. McNamara-Schroeder, R. F. Hennessey, G. A. Harding, R. C. Jensen, and W. E. Stumph
The Drosophila U1 and U6 Gene Proximal Sequence Elements Act as Important Determinants of the RNA Polymerase Specificity of Small Nuclear RNA Gene Promoters in Vitro and in Vivo
J. Biol. Chem., August 17, 2001; 276(34): 31786 - 31792.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.