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Nucleic Acids Research, Vol 24, Issue 22 4596-4597, Copyright © 1996 by Oxford University Press

ARTICLES

A cost effective base-matching assay with low backgrounds

X Su, G Mushinsky and AM Comeau
Theobald Smith Research Institute, Inc., The New England Regional Newborn Screening Program, Boston, MA 02130, USA. xsu@world.std.com

Base-matching or so-called mini-sequencing is a powerful technique for genotyping and mutation identification. However, its application is often hampered by high background and high cost. We have decreased the background by approximately 5-fold by incorporating an end-blocking step and using only 1/10 of the usual nucleotide concentrations.
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