Nucleic Acids Research, Vol 24, Issue 23 4791-4797, Copyright © 1996 by Oxford University Press
X Wang, PM Watt, EJ Louis, RH Borts and ID Hickson
Saccharomyces cerevisiae top2 mutants deficient in topoisomerase II
activity are defective in chromosome segregation during both mitotic and
meiotic cell divisions. To identify proteins that act in concert with
topoisomerase II during chromosome segregation in S.cerevisiae, we have
used a two-hybrid cloning approach. We report the isolation of the PAT1
gene (for protein associated with topoisomerase II), which encodes a novel
90 kDa proline- and glutamine-rich protein that interacts with a highly
conserved, leucine-rich region of topoisomerase II in vivo. Strains lacking
Pat1p exhibit a slow growth rate and a phenotype reminiscent of conditional
top2 mutants grown at the semi-permissive temperature; most notably, a
reduced fidelity of chromosome segregation during both mitosis and meiosis.
These findings indicate that the PAT1 gene is necessary for accurate
chromosome transmission during cell division in eukaryotic cells and
suggest that the interaction of Pat1p and topoisomerase II is an important
component of this function.
ARTICLES
Pat1: a topoisomerase II-associated protein required for faithful chromosome transmission in Saccharomyces cerevisiae
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
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