Nucleic Acids Research, Vol 24, Issue 24 4918-4923, Copyright © 1996 by Oxford University Press
ST Gregory, M O'Connor and AE Dahlberg
We have introduced the intervening sequence (IVS) from 23S rRNA of the rrnD
operon of Salmonella typhimurium into the equivalent position of
Escherichia coli 23S rRNA. Salmonella typhimurium 23S rRNA is fragmented
due to the RNase III-dependent removal of the approximately 100 nt
stem-loop structure that comprises the IVS. In this study, we have found
that insertion of the S. typhimurium IVS into E. coli 23S rRNA causes
fragmentation of the RNA but does not affect ribosome function. Cells
expressing the fragmented 23S rRNA exhibited wild-type growth rates.
Fragmented RNA was found in the actively translating polysome pool and did
not alter the sedimentation profile of ribosomal subunits, 70S ribosomes or
polysomes. Finally, hybrid 23S rRNA carrying the A2058G mutation conferred
high level erythromycin resistance indistinguishable from that of intact
23S rRNA carrying this mutation. These observations indicate that the
presence of this IVS and its removal are phenotypically silent. As observed
in an RNase III- deficient strain, processing of the IVS was not required
for the production of functional ribosomes.
ARTICLES
Functional Escherichia coli 23S rRNAs containing processed and unprocessed intervening sequences from Salmonella typhimurium
Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA. Steven_Gregory@Brown.edu
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