Nucleic Acids Research, Vol 24, Issue 24 4954-4961, Copyright © 1996 by Oxford University Press
C Sawa, M Goto, F Suzuki, H Watanabe, J Sawada and H Handa
Transcription factor E4TF1 is the human homolog of GABP and has been
renamed hGABP (human GABP). hGABP is composed of two types of subunits;
hGABP beta1/E4TF1-53 and the ets-related protein hGABP alpha/E4TF1-60. Both
bind together to form an (alpha)2(beta1)2 heterotetrameric complex on DNA
and activate transcription at specific promoters in vitro. Tetramer
formation depends on two regions of hGABP beta1; the N- terminal region
containing the Notch/ankyrin-type repeats is necessary for binding to hGABP
alpha and the C-terminal region is necessary for homodimerization. In this
report, we constructed various deletion mutants of hGABP beta1 in order to
delimit the functional regions required for nuclear localization and
transcription activity. We found that hGABP beta1 localization in the
nucleus is dependent on a region located between amino acids 243 and 330
and that the presence of hGABP beta1 influences the efficiency of hGABP
alpha transport into the nucleus. Next, we demonstrated that the hGABP
complex composed of alpha and beta1 subunits activates transcription from
the adenovirus early 4 promoter in vivo. This transcription activation
needs the C-terminal region of hGABP beta1 and is consistent with results
obtained with the in vitro assay. Furthermore, site-directed mutagenesis
analysis of the C-terminal region reveals that the alpha-helix structure
and the leucine residues are important for formation of a heterotetrameric
complex with hGABP alpha in vitro and for transcription activation in vivo.
These results suggest that hGABP beta1 stimulates transcription as part of
a heterotetrameric complex with hGABP alpha in vivo.
ARTICLES
Functional domains of transcription factor hGABP beta1/E4TF1-53 required for nuclear localization and transcription activation
Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama, Japan.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. E. Lucas, K. S. Crider, D. R. Powell, P. Kapoor-Vazirani, and P. M. Vertino Methylation-sensitive Regulation of TMS1/ASC by the Ets Factor, GA-binding Protein-{alpha} J. Biol. Chem., May 29, 2009; 284(22): 14698 - 14709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Xu and M. Komatsu Promoter Cloning and Characterization of the Anti-vascular Proliferation Gene, R-ras: ROLE OF ETS- AND SP-BINDING MOTIFS J. Biol. Chem., January 30, 2009; 284(5): 2706 - 2718. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-H. Xue, X. Jing, J. Bollenbacher-Reilley, D.-M. Zhao, J. S. Haring, B. Yang, C. Liu, G. A. Bishop, J. T. Harty, and W. J. Leonard Targeting the GA Binding Protein {beta}1L Isoform Does Not Perturb Lymphocyte Development and Function Mol. Cell. Biol., July 1, 2008; 28(13): 4300 - 4309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhang, M. Tuzova, Z.-X. J. Xiao, W. W. Cruikshank, and D. M. Center Pro-IL-16 Recruits Histone Deacetylase 3 to the Skp2 Core Promoter through Interaction with Transcription Factor GABP J. Immunol., January 1, 2008; 180(1): 402 - 408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Jaworski, C. L. Smith, and S. J. Burden GA-Binding Protein Is Dispensable for Neuromuscular Synapse Formation and Synapse-Specific Gene Expression Mol. Cell. Biol., July 1, 2007; 27(13): 5040 - 5046. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. T. Smith, R. D. Nicholls, and D. Reines The gene encoding the fragile X RNA-binding protein is controlled by nuclear respiratory factor 2 and the CREB family of transcription factors Nucleic Acids Res., February 25, 2006; 34(4): 1205 - 1215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Shimokawa and C. Ra C/EBP{alpha} functionally and physically interacts with GABP to activate the human myeloid IgA Fc receptor (Fc{alpha}R, CD89) gene promoter Blood, October 1, 2005; 106(7): 2534 - 2542. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Delehouzee, T. Yoshikawa, C. Sawa, J.-i. Sawada, T. Ito, M. Omori, T. Wada, Y. Yamaguchi, Y. Kabe, and H. Handa GABP, HCF-1 and YY1 are involved in Rb gene expression during myogenesis Genes Cells, July 1, 2005; 10(7): 717 - 731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sunesen, M. Huchet-Dymanus, M. O. Christensen, and J.-P. Changeux Phosphorylation-Elicited Quaternary Changes of GA Binding Protein in Transcriptional Activation Mol. Cell. Biol., November 15, 2003; 23(22): 8008 - 8018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sawa, T. Yoshikawa, F. Matsuda-Suzuki, S. Delehouzee, M. Goto, H. Watanabe, J.-i. Sawada, K. Kataoka, and H. Handa YEAF1/RYBP and YAF-2 Are Functionally Distinct Members of a Cofactor Family for the YY1 and E4TF1/hGABP Transcription Factors J. Biol. Chem., June 14, 2002; 277(25): 22484 - 22490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Hauck, R. G. Kaba, M. Lipp, R. Dietz, and R. von Harsdorf Regulation of E2F1-Dependent Gene Transcription and Apoptosis by the ETS-Related Transcription Factor GABP{gamma}1 Mol. Cell. Biol., April 1, 2002; 22(7): 2147 - 2158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tanaka, A. Ueda, H. Kanamori, H. Ideguchi, J. Yang, S. Kitajima, and Y. Ishigatsubo Cell-cycle-dependent Regulation of Human aurora A Transcription Is Mediated by Periodic Repression of E4TF1 J. Biol. Chem., March 15, 2002; 277(12): 10719 - 10726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Morii, H. Ogihara, K. Oboki, C. Sawa, T. Sakuma, S. Nomura, J. D. Esko, H. Handa, and Y. Kitamura Inhibitory effect of the mi transcription factor encoded by the mutant mi allele on GA binding protein-mediated transcript expression in mouse mast cells Blood, May 15, 2001; 97(10): 3032 - 3039. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Briguet and M. A. Ruegg The Ets Transcription Factor GABP Is Required for Postsynaptic Differentiation In Vivo J. Neurosci., August 15, 2000; 20(16): 5989 - 5996. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chinenov, M. Henzl, and M. E. Martin The alpha and beta Subunits of the GA-binding Protein Form a Stable Heterodimer in Solution. REVISED MODEL OF HETEROTETRAMERIC COMPLEX ASSEMBLY J. Biol. Chem., March 10, 2000; 275(11): 7749 - 7756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-i. Sawada, N. Simizu, F. Suzuki, C. Sawa, M. Goto, M. Hasegawa, T. Imai, H. Watanabe, and H. Handa Synergistic Transcriptional Activation by hGABP and Select Members of the Activation Transcription Factor/cAMP Response Element-binding Protein Family J. Biol. Chem., December 10, 1999; 274(50): 35475 - 35482. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hoque, K.-i. Ishizu, A. Matsumoto, S.-I. Han, F. Arisaka, M. Takayama, K. Suzuki, K. Kato, T. Kanda, H. Watanabe, et al. Nuclear Transport of the Major Capsid Protein Is Essential for Adeno-Associated Virus Capsid Formation J. Virol., September 1, 1999; 73(9): 7912 - 7915. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Yamaguchi, T. Wada, D. Watanabe, T. Takagi, J. Hasegawa, and H. Handa Structure and Function of the Human Transcription Elongation Factor DSIF J. Biol. Chem., March 19, 1999; 274(12): 8085 - 8092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Suzuki, M. Goto, C. Sawa, S. Ito, H. Watanabe, J.-i. Sawada, and H. Handa Functional Interactions of Transcription Factor Human GA-binding Protein Subunits J. Biol. Chem., November 6, 1998; 273(45): 29302 - 29308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Smith Jr., V. S. Carl, T. Lodie, and M. J. Fenton Secretory Interleukin-1 Receptor Antagonist Gene Expression Requires both a PU.1 and a Novel Composite NF-kappa B/PU.1/ GA-binding Protein Binding Site J. Biol. Chem., September 11, 1998; 273(37): 24272 - 24279. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Vassias, U. Hazan, Y. Michel, C. Sawa, H. Handa, L. Gouya, and F. Morinet Regulation of Human B19 Parvovirus Promoter Expression by hGABP (E4TF1) Transcription Factor J. Biol. Chem., April 3, 1998; 273(14): 8287 - 8293. [Abstract] [Full Text] [PDF]
|
|