Nucleic Acids Research, Vol 24, Issue 4 558-565, Copyright © 1996 by Oxford University Press
LA Harrington and BJ Andrews
In Saccharomyces cerevisiae commitment to cell division occurs late in the
G1 phase of the cell cycle at a point called Start and requires the
activity of the Cdc28 protein kinase and its associated G1 cyclins. The
Swi4,6-dependent cell cycle box binding factor, SBF, is important for
maximal expression of the G1 cyclin and HO endonuclease genes at Start. The
cell cycle regulation of these genes is modulated through an upstream
regulatory element termed the SCB (Swi4,6-dependent cell cycle box,
CACGAAA), which is dependent on both SW14 and SW16. Although binding of
Swi4 and Swi6 to SCB sequences has been well characterized in vitro, the
binding of SBF in vivo has not been examined. We used in vivo dimethyl
sulfate footprinting to examine the occupancy of SCB sequences throughout
the cell cycle. We found that binding to SCB sequences occurred in the G1
phase of the cell cycle and was greatly reduced in G2. In the absence of
either Swi4 or Swi6, SCB sequences were not occupied at any cell cycle
stage. These results suggest that the G1-specific expression of
SCB-dependent genes is regulated at the level of DNA binding in vivo.
ARTICLES
Binding to the yeast Swi4,6-dependent cell cycle box, CACGAAA, is cell cycle regulated in vivo
Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada.
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