Nucleic Acids Research, Vol 24, Issue 4 619-627, Copyright © 1996 by Oxford University Press
M Gniadkowski, M Hemmings-Mieszczak, U Klahre, HX Liu and W Filipowicz
Introns of nuclear pre-mRNAs in dicotyledonous plants, unlike introns in
vertebrates or yeast, are distinctly rich in A+U nucleotides and this
feature is essential for their processing. In order to define more
precisely sequence elements important for intron recognition in plants, we
investigated the effects of short insertions, either U-rich or A- rich, on
splicing of synthetic introns in transfected protoplast of Nicotiana
plumbaginifolia. It was found that insertions of U-rich (sequence UUUUUAU)
but not A-rich (AUAAAAA) segments can activate splicing of a GC-rich
synthetic infron, and that U-rich segments, or multimers thereof, can
function irrespective of the site of insertion within the intron.
Insertions of multiple U-rich segments, either at the same or different
locations, generally had an additive, stimulatory effect on splicing.
Mutational analysis showed that replacement of one or two U residues in the
UUUUUAU sequence with A or C residues had only a small effect on splicing,
but replacement with G residues was strongly inhibitory. Proteins that
interact with fragments of natural and synthetic pre-mRNAs in vitro were
identified in nuclear extracts of N.plumbaginifolia by UV cross- linking.
The profile of cross-linked plant proteins was considerably less complex
than that obtained with a HeLa cell nuclear extract. Two major
cross-linkable plant proteins had apparent molecular mass of 50 and 54 kDa
and showed affinity for oligouridilates present in synGC introns or for
poly(U).
ARTICLES
Characterization of intronic uridine-rich sequence elements acting as possible targets for nuclear proteins during pre-mRNA splicing in Nicotiana plumbaginifolia
Friedrich Miescher Institute, Ch-4002 Basel, Switzerland.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Touchon, A. Arneodo, Y. d'Aubenton-Carafa, and C. Thermes Transcription-coupled and splicing-coupled strand asymmetries in eukaryotic genomes Nucleic Acids Res., September 23, 2004; 32(17): 4969 - 4978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Lewandowska, C. G. Simpson, G. P. Clark, N. S. Jennings, M. Barciszewska-Pacak, C.-F. Lin, W. Makalowski, J. W.S. Brown, and A. Jarmolowski Determinants of Plant U12-Dependent Intron Splicing Efficiency PLANT CELL, May 1, 2004; 16(5): 1340 - 1352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Lopato, C. Forstner, M. Kalyna, J. Hilscher, U. Langhammer, K. Indrapichate, Z. J. Lorkovic', and A. Barta Network of Interactions of a Novel Plant-specific Arg/Ser-rich Protein, atRSZ33, with atSC35-like Splicing Factors J. Biol. Chem., October 11, 2002; 277(42): 39989 - 39998. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. H. L. Lambermon, Y. Fu, D. A. W. Kirk, M. Dupasquier, W. Filipowicz, and Z. J. Lorkovic UBA1 and UBA2, Two Proteins That Interact with UBP1, a Multifunctional Effector of Pre-mRNA Maturation in Plants Mol. Cell. Biol., June 15, 2002; 22(12): 4346 - 4357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Rose and J. A. Beliakoff Intron-Mediated Enhancement of Gene Expression Independent of Unique Intron Sequences and Splicing Plant Physiology, February 1, 2000; 122(2): 535 - 542. [Abstract] [Full Text]
|
|
|
|
|
|
M. Golovkin and A. S. N. Reddy An SC35-like Protein and a Novel Serine/Arginine-rich Protein Interact with Arabidopsis U1-70K Protein J. Biol. Chem., December 17, 1999; 274(51): 36428 - 36438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lal, J.-H. Choi, and L. C. Hannah The AG Dinucleotide Terminating Introns Is Important but Not Always Required for Pre-mRNA Splicing in the Maize Endosperm Plant Physiology, May 1, 1999; 120(1): 65 - 72. [Abstract] [Full Text]
|
|
|
|
|
|
C. Domon, Z. J. Lorkovic, J. Valcarcel, and W. Filipowicz Multiple Forms of the U2 Small Nuclear Ribonucleoprotein Auxiliary Factor U2AF Subunits Expressed in Higher Plants J. Biol. Chem., December 18, 1998; 273(51): 34603 - 34610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. W. M. Sablowski and E. M. Meyerowitz Temperature-Sensitive Splicing in the Floral Homeotic Mutant apetala3-1 PLANT CELL, September 1, 1998; 10(9): 1453 - 1464. [Abstract] [Full Text]
|
|