Nucleic Acids Research, Vol 24, Issue 4 628-639, Copyright © 1996 by Oxford University Press
H Hilbert, R Himmelreich, H Plagens and R Herrmann
To sequence the entire 800 kilobase pair genome of the bacterium Mycoplasma
pneumoniae, a plasmid library was established with contained the majority
of the EcoR1 fragments from M.pneumoniae. The EcoR1 fragments were
subcloned from an ordered cosmid library comprising the complete
M.pneumoniae genome. Individual plasmid clones were sequenced in an ordered
fashion mainly by primer walking. We report here the initial results from
the sequence analysis of -56 kb comprising the dnaA region as a potential
origin of replication, the ATPase operon and a region coding for a cluster
of ribosomal protein genes. The data were compared with the corresponding
genes/operons from Bacillus subtilis, Escherichia coli, Mycoplasma
capricolum and Mycoplasma gallisepticum.
ARTICLES
Sequence analysis of 56 kb from the genome of the bacterium Mycoplasma pneumoniae comprising the dnaA region, the atp operon and a cluster of ribosomal protein genes
Zentrum fur Molekulare Biologie Heidelberg, Universitat Heidelberg, Germany.
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