Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (122K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Patnaik, P.
Right arrow Articles by Cross, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Patnaik, P.
Right arrow Articles by Cross, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 24, Issue 4 668-675, Copyright © 1996 by Oxford University Press

ARTICLES

Artificial linear mini-chromosomes for Trypanosoma brucei

PK Patnaik, N Axelrod, LHT Van der Ploeg and GAM Cross
Laboratory of Molecular Parasitology, The Rockefeller University, New York, NY 10021-6399 USA.

We have constructed artificial linear mini- chromosomes for the parasitic protozoan Trypanosoma brucei. These chromosomes exist at approx. 2 copies per cell, are indefinitely stable under selection but are lost from 50% of the transformed population in approx. 7 generations when grown in the absence of selective pressure. Consistent with results obtained earlier with natural chromosomes in T.brucei, the telomeres on these artificial chromosomes grow, adding approx. 1- 1.5 telomeric repeats per generation. The activity of a procyclic acidic repetitive protein (parp) gene promoter on these elements is unaffected by its proximity to a telomere, implying the lack of a telomere- proximal position effect (TPE) in procyclic trypanosomes. Among other things, these autonomously replicating dispensable genetic elements will provide a defined system for the study of nuclear DNA replication, karyotypic plasticity and other aspects of chromosomal behavior in this ancient eukaryotic lineage.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
P. Dubessay, C. Ravel, P. Bastien, M.-F. Lignon, B. Ullman, M. Pages, and C. Blaineau
Effect of large targeted deletions on the mitotic stability of an extra chromosome mediating drug resistance in Leishmania
Nucleic Acids Res., August 1, 2001; 29(15): 3231 - 3240.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Tamar and B. Papadopoulou
A Telomere-mediated Chromosome Fragmentation Approach to Assess Mitotic Stability and Ploidy Alterations of Leishmania Chromosomes
J. Biol. Chem., April 6, 2001; 276(15): 11662 - 11673.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.