Nucleic Acids Research, Vol 24, Issue 4 688-693, Copyright © 1996 by Oxford University Press
JA Kowalak, E Bruenger, T Hashizume, JM Peltier, J Ofengand and JA McCloskey
Mass spectrometry-based methods have been used to study post-
transcriptional modification in the 1900-1974 nt segment of domain IV in
23S rRNA of Escherichia coli, a region which interacts with domain V in
forming the three- dimensional structure of the peptidyl transferase center
within the ribosome. A nucleoside constituent of M r 258 (U*)which occurs
at position 1915, within the highly modified oligonucleotide sequence
1911-psiAACU*Apsi-1917, was characterized as 3- methylpseudouridine
(m3psi). The assignment was confirmed by chemical synthesis of m3psi and
comparison with the natural nucleoside by liquid chromatography-mass
spectrometry. 3-Methylpseudouridine is previously unknown in nature and is
the only known derivative of the common modified nucleoside pseudouridine
thus far found in bacterial rRNA.
ARTICLES
Structural characterization of U*-1915 in domain IV from Escherichia coli 23S ribosomal RNA as 3-methylpseudouridine
Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA.
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