Nucleic Acids Research, Vol 24, Issue 5 931-937, Copyright © 1996 by Oxford University Press
YF Wei, KC Carter, RP Wang and BK Shell
The Escherichia coli AlkB protein is involved in protecting cells against
mutation and cell death induced specifically by SN2-type alkylating agents
such as methyl methanesulfonate (MMS). A human cDNA encoding a polypeptide
homologous to E.coli AlkB was discovered by searching a database of
expressed sequence tags (ESTs) derived from high throughput cDNA
sequencing. The full-length human AlkB homolog (hABH) cDNA clone contains a
924 bp open reading frame encoding a 34 kDa protein which is 52% similar
and 23% identical to E.coli AlkB. The hABH gene, which maps to chromosome
14q24, was ubiquitously expressed in 16 human tissues examined. When hABH
was expressed in E.coli alkB mutant cells partial rescue of the cells from
MMS-induced cell death occurred. Under the conditions used expression of
hABH in skin fibroblasts was not regulated by treatment with MMS. Our
findings show that the AlkB protein is structurally and functionally
conserved from bacteria to human, but its regulation may have diverged
during evolution.
ARTICLES
Molecular cloning and functional analysis of a human cDNA encoding an Escherichia coli AlkB homolog, a protein involved in DNA alkylation damage repair
Department of Molecular Biology, Human Genome Sciences Inc., Rockville, MD 20850-3338, USA.
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