Nucleic Acids Research, Vol 24, Issue 6 1037-1044, Copyright © 1996 by Oxford University Press
JR Maddock, J Roy and JL Woolford Jr
We have identified six new genes whose products are necessary for the
splicing of nuclear pre-mRNA in the yeast Saccharomyces cerevisiae. A
collection of 426 temperature-sensitive yeast strains was generated by EMS
mutagenesis. These mutants were screened for pre-mRNA splicing defects by
an RNA gel blot assay, using the intron- containing CRY1 and ACT1 genes as
hybridization probes. We identified 20 temperature- sensitive mutants
defective in pre-mRNA splicing. Twelve appear to be allelic to the
previously identified prp2, prp3, prp6, prp16/prp23, prp18, prp19 or prp26
mutations that cause defects in spliceosome assembly or the first or second
step of splicing. One is allelic to SNR14 encoding U4 snRNA. Six new
complementation groups, prp29-prp34, were identified. Each of these mutants
accumulates unspliced pre-mRNA at 37 degrees C and thus is blocked in
spliceosome assembly or early steps of pre-mRNA splicing before the first
cleavage and ligation reaction. The prp29 mutation is suppressed by
multicopy PRP2 and displays incomplete patterns of complementation with
prp2 alleles, suggesting that the PRP29 gene product may interact with that
of PRP2. There are now at least 42 different gene products, including the
five spliceosomal snRNAs and 37 different proteins that are necessary for
pre-mRNA splicing in Saccharomyces cerevisiae. However, the number of yeast
genes identifiable by this approach has not yet been exhausted.
ARTICLES
Six novel genes necessary for pre-mRNA splicing in Saccharomyces cerevisiae
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213 USA.
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