Nucleic Acids Research, Vol 24, Issue 6 1047-1051, Copyright © 1996 by Oxford University Press
M Lnenicek-Allen, CM Read and C Crane-Robinson
The HMG box of human LEF-1 (hLEF-1, formerly TCF1alpha) has been expressed
in four forms: a parent box of 81 amino acids and constructs having either
a 10 amino acid C-terminal extension, a 9 amino acid N- terminal extension,
or both. These four species have been compared for DNA binding and bending
ability using a 28 bp recognition sequence from the TCR alpha-chain
enhancer. In the bending assay, whereas the parent box and that with the
N-terminal extension bent the DNA by 57/58 degrees, the box extended at the
C-terminus bent the DNA by 77/78 degrees, irrespective of the presence or
absence of the N-terminal extension. A 6- fold increase in DNA affinity
also resulted from addition of both terminal extensions. These observations
redefine the functional boundaries of the HMG box. The structure of a mouse
LEF- 1/DNA complex recently published [Love et al. (1995) Nature 376, 791-
795] implies that the higher DNA affinity and in particular the increased
bend angle observed are consequences, at least in part, of the C-terminal
extension spanning the major groove on the inside of the DNA bend.
ARTICLES
The DNA bend angle and binding affinity of an HMG box increased by the presence of short terminal arms
Biophysics Laboratories, University of Portsmouth, United Kingdom.
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