Nucleic Acids Research, Vol 24, Issue 6 1112-1118, Copyright © 1996 by Oxford University Press
RM Williams, VA Rhodius, AI Bell, A Kolb and SJ Busby
At class II CRP-dependent promoters the DNA site for CRP overlaps the DNA
site for RNA polymerase, covering the -35 region. Transcription activation
at class II CRP- dependent promoters requires a contact between an
activating region in the upstream subunit of the bound CRP dimer and a
contact site in the C-terminal domain of the alpha-subunit of RNA
polymerase. Transcription activation is suppressed by amino acid
substitutions in the activating region, but activation can be restored by
second site substitutions at K52 or E96. These substitutions identify two
separate regions on the surface of CRP that appear to be able to interact
with RNA polymerase specifically at class II promoters. Using the method of
'oriented heterodimers' we show that these alternative activating regions
are functional in the downstream subunit of the bound CRP dimer.
ARTICLES
Orientation of functional activating regions in the Escherichia coli CRP protein during transcription activation at class II promoters
School of Biochemistry, University of Birmingham, United Kingdom.
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