Nucleic Acids Research, Vol 24, Issue 6 1158-1163, Copyright © 1996 by Oxford University Press
W Huang, JM Wong and E Bateman
Eukaryotic promoter elements specify the direction and efficiency of
transcription, as well as the type of RNA polymerase to be used. One such
element, the TATA box, is thought to participate in determining the
direction of transcription and can function within promoters for RNA
polymerase II or III, depending on the sequence context. In this report the
ability of four different TATA boxes to support transcription in vitro was
determined. It was found that TATA elements are not directional. However,
they support transcription by RNA polymerases II and III. An upstream
activating sequence was found to stimulate downstream transcription by RNA
polymerase II and to inhibit upstream transcription by RNA polymerases II
and III. Thus a promoter necessarily consists of a TATA element and
upstream sequences in order to specify the direction of transcription and
the type of polymerase to be used.
ARTICLES
TATA elements direct bi-directional transcription by RNA polymerases II and III
Department of Microbiology, University of Vermont, Burlington 05405 USA.
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