Nucleic Acids Research, Vol 24, Issue 6 1164-1170, Copyright © 1996 by Oxford University Press
EM Weidenhammer, M Singh, M Ruiz-Noriega and JL Woolford Jr
The pre-mRNA splicing factor Prp31p was identified in a screen of
temperature-sensitive yeast strains for those exhibiting a splicing defect
upon shift to the non- permissive temperature. The wild-type PRP31 gene was
cloned and shown to be essential for cell viability. The PRP31 gene is
predicted to encode a 60 kDa polypeptide. No similarities with other known
splicing factors or motifs indicative of protein- protein or RNA-protein
interaction domains are discernible in the predicted amino acid sequence. A
PRP31 allele bearing a triple repeat of the hemagglutinin epitope has been
generated. The tagged protein is functional in vivo and a single
polypeptide species of the predicted size was detected by Western analysis
with proteins from yeast cell extracts. Functional Prp31p is required for
the processing of pre-mRNA species both in vivo and in vitro, indicating
that the protein is directly involved in the splicing pathway.
ARTICLES
The PRP31 gene encodes a novel protein required for pre-mRNA splicing in Saccharomyces cerevisiae
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213 USA.
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