Nucleic Acids Research, Vol 24, Issue 7 1322-1329, Copyright © 1996 by Oxford University Press
JC Jackson and JM Lopes
In Saccharomyces cerevisiae, regulation of the phospholipid biosynthetic
genes, INO1, CHO1, CHO2 and OPI3, is known to occur at the level of
transcript abundance. Derepression in response to inositol deprivation
requires the INO2 and INO4 regulatory genes. Repression in response to
inositol supplementation requires the OPI1 regulatory gene. Here, we
examined the role of the UME6 global negative regulatory gene in expression
of the phospholipid biosynthetic genes. These studies were stimulated by
the finding that the INO1 promoter included a UME6 cognate cis-acting
regulatory sequence (URS1). We found that the UME6 negative regulatory gene
was involved in regulation of phospholipid biosynthetic gene expression
through two distinct regulatory pathways. One pathway was the direct
repression of INO1 expression through the URS1 element. Surprisingly, the
UME6 gene was also required for derepression of CHO1, CHO2 and OPI3 gene
expression. Consistent with this observation, the UME6 gene was required
for wild-type levels of expression of the INO2 positive regulatory gene.
Therefore, the UME6 gene has both a negative and a positive role in
regulating phospholipid biosynthesis.
ARTICLES
The yeast UME6 gene is required for both negative and positive transcriptional regulation of phospholipid biosynthetic gene expression
Department of Molecular and Cellular Biochemistry, Loyola University of Chicago, Maywood, IL 60153, USA.
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