Nucleic Acids Research, Vol 24, Issue 8 1443-1452, Copyright © 1996 by Oxford University Press
MC Huber, G Kruger and C Bonifer
The chicken lysozyme locus is gradually activated during macrophage
development exhibiting a specific chromatin structure with each
differentiation state. Its small size and the extensive characterization of
its cis-regulatory elements allows us to study even subtle changes in
chromatin structure of the entire gene locus during transcriptional
activation. Tissue-specific and position independent expression of the
lysozyme locus in transgenic mice requires the cooperation of all
cis-regulatory elements. In order to elucidate further the molecular basis
of locus activation, we have determined nucleosome positions within the
complete 5'-regulatory region of the chicken lysozyme locus in chicken
myeloid cell lines and transgenic mice. Each cis-regulatory element
develops its unique nucleosomal structure and each one remodels chromatin
differently. The nucleosomal organization of the endogenous gene in chicken
cell lines and the transgene in the mouse turned out to be identical,
enabling us to study the influence of cis-regulatory deletions on the
development of an active chromatin structure in transgenic mice. Transgenes
with a deletion of an important cis-regulatory element show an impediment
in nucleosome reorganization as compared with the complete lysozyme locus.
We demonstrate that multicopy transgene-clusters in position dependently
expressing mouse lines exhibit a heterogeneous chromatin organization.
ARTICLES
Genomic position effects lead to an inefficient reorganization of nucleosomes in the 5'-regulatory region of the chicken lysozyme locus in transgenic mice
Institut fur Biologie 111 der Universitat Freiburg, Germany.
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