Nucleic Acids Research, Vol 24, Issue 8 1489-1496, Copyright © 1996 by Oxford University Press
JP Pais de Barros, G Keith, C El Adlouni, AL Glasser, G Mack, G Dirheimer and J Desgres
The nucleotide analysis of a cytoplasmic tRNA(Leu) isolated from bovine
liver revealed the presence of an unknown modified nucleotide N. The
corresponding N nucleoside was isolated by different enzymatic and
chromatographic protocols from a partially purified preparation of this
tRNA(Leu). Its chemical characterization was determined from its
chromatographic properties, UV-absorption spectroscopy and mass
spectrometric measurements, as well as from those of the borohydride
reduced N nucleoside and its etheno-trimethylsilyl derivative. The
structure of N was established as 2'-O-methyl-5-formylcytidine (f5CM), and
its reduced derivative as 2'-O-methyl-5-hydroxy-methylcytidine (om5Cm). By
sequencing the bovine liver tRNA(Leu), the structure of the anticodon was
determined as f5CmAA. In addition, the nucleotide sequence showed two
primary structures differing only by the nucleotide 47c which is either
uridine or adenosine. The two slightly differing bovine liver
tRNAs-Leu(f5CmAA) are the only tRNAs so far sequenced which contain f5Cm.
The role of such a modified cytidine at the first position of the anticodon
is discussed in terms of decoding properties for the UUG and UUA leucine
codons. Recently, precise evidence was obtained for the presence of f5Cm at
the same position in tRNAs(Leu)(NAA) isolated from rabbit and lamb liver.
Therefore, the 2'- O-methyl-5-formyl modification of cytidine at position
34 could be a general feature of cytoplasmic tRNAs(Leu)(NAA) in mammals.
ARTICLES
2'-O-methyl-5-formylcytidine (f5Cm), a new modified nucleotide at the 'wobble' of two cytoplasmic tRNAs Leu (NAA) from bovine liver
Laboratoire de Biochimie Medicale, Equipe de Recherche Biochimie- Biologie Cellulaire, Faculte de Medecine et Centre Hospitalier Universitaire, Universite de Bourgogne, Dijon, France.
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