Nucleic Acids Research, Vol 24, Issue 8 1540-1546, Copyright © 1996 by Oxford University Press
KS Sweder, R Chun, T Mori and PC Hanawalt
Several proteins, including Rad3 and Rad25(Ssl2), are essential for
nucleotide excision repair (NER) and function in the RNA polymerase II
transcription initiation complex TFIIH. Mutations in genes encoding two
other subunits of TFIIH, TFB1 and SSL1, result in UV sensitivity and have
been shown to take part in NER in an in vitro system. However, a deficiency
in global NER does not exclude the possibility that such repair-deficient
mutants can perform transcription-coupled repair (TCR), as shown for
xeroderma pigmentosum group C. To date, temperature- sensitive C-terminal
truncations of Tfbl are the only TFIIH mutations that result in
intermediate UV sensitivity, which might indicate a deficiency in either
the global NER or TCR pathways. We have directly analyzed both TCR and
global NER in these mutants. We found that ssl1, rad3 and tfb1 mutants,
like rad25(ssl2-xp) mutants, are deficient in both the global NER and TCR
pathways. Our results support the view that the mutations in any one of the
genes encoding subunits of TFIIH result in deficiencies in both global and
TCR pathways of NER. We suggest that when subunits of TFIIH are in limiting
amounts, TCR may preclude global NER.
ARTICLES
DNA repair deficiencies associated with mutations in genes encoding subunits of transcription initiation factor TFIIH in yeast
Department of Biological Sciences, Stanford University, CA 94305-5020, USA.
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