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Nucleic Acids Research, Vol 24, Issue 9 1608-1615, Copyright © 1996 by Oxford University Press

ARTICLES

The polypyrimidine tract binding (PTB) protein interacts with single- stranded DNA in a sequence-specific manner

F Brunel, MM Zakin, H Buc and M Buckle
Unite d'Expression des Genes Eucaryotes, Institut Pasteur, Paris, France.

Polypyrimidine tract binding (PTB) protein is a cellular factor whose function is unknown. Various RNA or single-stranded DNA sequences have been shown to interact with PTB. In this paper, using laser UV crosslinking and electrophoretic mobility shift assays to probe DNA- protein interactions, we demonstrate that PTB binding at a single- stranded DNA target is highly sequence-specific. We provide data showing that PTB interacts with the top strand of the adenovirus major late promoter transcriptional initiator, a sequence rich in pyrimidine residues. We also demonstrate that PTB is organised into at least two different binding domains.
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