Nucleic Acids Research, Vol 24, Issue 9 1695-1701, Copyright © 1996 by Oxford University Press
TH Huang, T Oka, T Asai, T Okada, BW Merrills, PN Gertson, RH Whitson and K Itakura
We detected a novel nuclear protein, MRF, that binds to multiple sites on
the modulator which is located upstream of the human cytomegalovirus major
immediate early gene enhancer. The expression of MRF is differentiation
specific; the DNA binding activity is present in nuclear extracts from
undifferentiated Tera-2 and THP-1 cells, but significantly reduced after
these cells are induced to differentiate. In undifferentiated cells the
enhancer activity is repressed by the modulator and upon differentiation
the enhancer becomes active. Competitive binding assays demonstrate that
MRF requires the presence of multiple A+T stretches for binding to DNA,
rather than binding to a specific DNA sequence. Mutations of these
stretches in the modulator reduce the binding activity of MRF, as well as
the repressing activity on the enhancer. These results suggest that MRF may
act as a repressor of enhancer function. We propose that MRF binds over the
entire modulator and exerts repressor activity.
ARTICLES
Repression by a differentiation-specific factor of the human cytomegalovirus enhancer
Department of Molecular Genetics, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Pasini, K. H. Hansen, J. Christensen, K. Agger, P. A.C. Cloos, and K. Helin Coordinated regulation of transcriptional repression by the RBP2 H3K4 demethylase and Polycomb-Repressive Complex 2 Genes & Dev., May 15, 2008; 22(10): 1345 - 1355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yamakawa, R. H. Whitson, S.-L. Li, and K. Itakura Modulator Recognition Factor-2 Is Required for Adipogenesis in Mouse Embryo Fibroblasts and 3T3-L1 Cells Mol. Endocrinol., February 1, 2008; 22(2): 441 - 453. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. G. Scibetta, S. Santangelo, J. Coleman, D. Hall, T. Chaplin, J. Copier, S. Catchpole, J. Burchell, and J. Taylor-Papadimitriou Functional Analysis of the Transcription Repressor PLU-1/JARID1B Mol. Cell. Biol., October 15, 2007; 27(20): 7220 - 7235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. T. Saffert and R. F. Kalejta Human Cytomegalovirus Gene Expression Is Silenced by Daxx-Mediated Intrinsic Immune Defense in Model Latent Infections Established In Vitro J. Virol., September 1, 2007; 81(17): 9109 - 9120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Sinclair and P. Sissons Latency and reactivation of human cytomegalovirus J. Gen. Virol., July 1, 2006; 87(7): 1763 - 1779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Reeves, P. A. MacAry, P. J. Lehner, J. G. P. Sissons, and J. H. Sinclair Latency, chromatin remodeling, and reactivation of human cytomegalovirus in the dendritic cells of healthy carriers PNAS, March 15, 2005; 102(11): 4140 - 4145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Wright, M. Bain, L. Teague, J. Murphy, and J. Sinclair Ets-2 repressor factor recruits histone deacetylase to silence human cytomegalovirus immediate-early gene expression in non-permissive cells J. Gen. Virol., March 1, 2005; 86(3): 535 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Patsialou, D. Wilsker, and E. Moran DNA-binding properties of ARID family proteins Nucleic Acids Res., January 7, 2005; 33(1): 66 - 80. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. McNees, J. A. Mahr, D. Ornelles, and L. R. Gooding Postinternalization Inhibition of Adenovirus Gene Expression and Infectious Virus Production in Human T-Cell Lines J. Virol., July 1, 2004; 78(13): 6955 - 6966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bain, M. Mendelson, and J. Sinclair Ets-2 Repressor Factor (ERF) mediates repression of the human cytomegalovirus major immediate-early promoter in undifferentiated non-permissive cells J. Gen. Virol., January 1, 2003; 84(1): 41 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shaham and C. I. Bargmann Control of neuronal subtype identity by the C. elegans ARID protein CFI-1 Genes & Dev., April 15, 2002; 16(8): 972 - 983. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Wilsker, A. Patsialou, P. B. Dallas, and E. Moran ARID Proteins: A Diverse Family of DNA Binding Proteins Implicated in the Control of Cell Growth, Differentiation, and Development Cell Growth Differ., March 1, 2002; 13(3): 95 - 106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Beisser, L. Laurent, J.-L. Virelizier, and S. Michelson Human Cytomegalovirus Chemokine Receptor Gene US28 Is Transcribed in Latently Infected THP-1 Monocytes J. Virol., July 1, 2001; 75(13): 5949 - 5957. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Meier Reactivation of the Human Cytomegalovirus Major Immediate-Early Regulatory Region and Viral Replication in Embryonal NTera2 Cells: Role of Trichostatin A, Retinoic Acid, and Deletion of the 21-Base-Pair Repeats and Modulator J. Virol., February 15, 2001; 75(4): 1581 - 1593. [Abstract] [Full Text]
|
|
|
|
|
|
P. B. Dallas, S. Pacchione, D. Wilsker, V. Bowrin, R. Kobayashi, and E. Moran The Human SWI-SNF Complex Protein p270 Is an ARID Family Member with Non-Sequence-Specific DNA Binding Activity Mol. Cell. Biol., May 1, 2000; 20(9): 3137 - 3146. [Abstract] [Full Text]
|
|
|
|
|
|
C. A. Lundquist, J. L. Meier, and M. F. Stinski A Strong Negative Transcriptional Regulatory Region between the Human Cytomegalovirus UL127 Gene and the Major Immediate-Early Enhancer J. Virol., November 1, 1999; 73(11): 9039 - 9052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Aiba-Masago, S. Baba, R.-Y. Li, Y. Shinmura, I. Kosugi, Y. Arai, M. Nishimura, and Y. Tsutsui Murine Cytomegalovirus Immediate-Early Promoter Directs Astrocyte-Specific Expression in Transgenic Mice Am. J. Pathol., March 1, 1999; 154(3): 735 - 743. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Shandala, R. Kortschak, S Gregory, and R Saint The Drosophila dead ringer gene is required for early embryonic patterning through regulation of argos and buttonhead expression Development, January 10, 1999; 126(19): 4341 - 4349. [Abstract] [PDF]
|
|