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Nucleic Acids Research, Vol 24, Issue 9 1747-1752, Copyright © 1996 by Oxford University Press

ARTICLES

Identification of promoter and stringent regulation of transcription of the fabH, fabD and fabG genes encoding fatty acid biosynthetic enzymes of Escherichia coli

SM Podkovyrov and TJ Larson
Department of Biochemistry and Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg, 24061-0308, USA.

In Escherichia coli, amino acid starvation results in the coordinate inhibition of a variety of metabolic activities, including fatty acid and phospholipid biosynthesis. By using primer extension analysis we identified the fabH promoter responsible for transcription of the fabH, fabD and fabG genes encoding fatty acid biosynthetic enzymes. The response of the fabH promoter to amino acid starvation was determined in vivo. Transcripts originating from the fabH promoter were quantified by employing a ribonuclease protection assay. The fabH promoter was subject to relA-dependent stringent control and was repressed approximately 4-fold upon amino acid starvation. The results suggest that inhibition of transcription initiation of lipid biosynthetic genes in starved cells contributes to the stringent control of lipid biosynthesis.
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