Nucleic Acids Research, Vol 25, Issue 10 1897-1902, Copyright © 1997 by Oxford University Press
N Murata-Kamiya, H Kamiya, H Kaji and H Kasai
Glyoxal is a major product of DNA oxidation in which Fenton-type oxygen
free radical-forming systems are involved. To determine the mutation
spectrum of glyoxal in mammalian cells and to compare the spectrum with
those observed in other experimental systems, we analyzed mutations in a
bacterial suppressor tRNA gene (supF) in the shuttle vector plasmid pMY189.
We treated pMY189 with glyoxal and immediately transfected it into simian
COS-7 cells. The cytotoxicity and mutation frequency increased according to
the dose of glyoxal. The majority of glyoxal- induced mutations (48%) were
single-base substitutions. Eighty three percent of the single-base
substitutions occurred at G:C base pairs. Among them, G:C-->T:A
transversions were predominant, followed by G:C-- >C:G transversions and
G:C-->A:T transitions. A:T-->T:A transversions were also observed.
Mutational hotspots within the supF gene were detected. These results
suggest that glyoxal may play an important role in mutagenesis induced by
oxygen free radicals.
ARTICLES
Glyoxal, a major product of DNA oxidation, induces mutations at G:C sites on a shuttle vector plasmid replicated in mammalian cells
Department of Health Policy and Management, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807, Japan.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. De Bont and N. van Larebeke Endogenous DNA damage in humans: a review of quantitative data Mutagenesis, May 1, 2004; 19(3): 169 - 185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nakayama, Y. Noguchi, T. Mori, S. Morisawa, and T. Yagi Comparison of mutagenic potentials and mutation spectra of benzene metabolites using supF shuttle vectors in human cells Mutagenesis, March 1, 2004; 19(2): 91 - 97. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Satou, H. Harashima, and H. Kamiya Mutagenic effects of 2-hydroxy-dATP on replication in a HeLa extract: induction of substitution and deletion mutations Nucleic Acids Res., May 15, 2003; 31(10): 2570 - 2575. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.D. Lewis, J.S. Harvey, E.M. Waters, D.O.F. Skibinski, and J.M. Parry Spontaneous mutation spectra in supF: comparative analysis of mammalian cell line base substitution spectra Mutagenesis, November 1, 2001; 16(6): 503 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Murata-Kamiya and H. Kamiya Methylglyoxal, an endogenous aldehyde, crosslinks DNA polymerase and the substrate DNA Nucleic Acids Res., August 15, 2001; 29(16): 3433 - 3438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kamiya and H. Kasai 2-Hydroxy-dATP is incorporated opposite G by Escherichia coli DNA polymerase III resulting in high mutagenicity Nucleic Acids Res., April 1, 2000; 28(7): 1640 - 1646. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ruiz-Laguna and C. Pueyo Hydrogen peroxide and coffee induce G:C->T:A transversions in the lacI gene of catalase-defective Escherichia coli Mutagenesis, January 1, 1999; 14(1): 95 - 102. [Abstract] [Full Text] [PDF]
|
|