Nucleic Acids Research, Vol 25, Issue 10 2035-2036, Copyright © 1997 by Oxford University Press
F Beranger, S Aresta, J de Gunzburg and J Camonis
Two-hybrid methods detect interactions between two proteins fused at the
C-termini of, respectively, a DNA-binding domain and the activation domain
of a transcriptional activator. Thus the N-terminus of none of these
proteins is available for interaction. We have tested whether a bait
protein with a reverted polarity (i.e. N-bait-LexA-C) is suitable for
two-hybrid interaction. We show that such constructs give a specific
interaction signal, and document two cases where the sensitivity is
dramatically increased. Such constructs might lead to the identification of
partners missed during classical two-hybrid screens.
ARTICLES
Getting more from the two-hybrid system: N-terminal fusions to LexA are efficient and sensitive baits for two-hybrid studies
INSERM U-248, Institut Curie, 26 rue d'Ulm, 75231 Paris cedex 05, France.
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