Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (41K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (6)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lankiewicz, S.
Right arrow Articles by Hatt, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lankiewicz, S.
Right arrow Articles by Hatt, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 25, Issue 10 2037-2038, Copyright © 1997 by Oxford University Press

ARTICLES

Enhanced RACE method using specific enrichment by biotinylated oligonucleotides bound to streptavidin coated magnetic particles

S Lankiewicz, G Gisselmann and H Hatt
Lehrstuhl fur Zellphysiologie, Ruhr-Universitat Bochum, Universitatsstrasse 150, D-44780 Bochum, Germany.

RACE (rapid amplification of cDNA ends) is commonly used for identification and isolation of 3'and 5'termini of cDNA. We developed an improvement of the RACE-method that allows the enrichment of wanted fragments. The important new feature is the purification of the amplified products by biotinylated oligonucleotides that hybridize internally. Hybrids are isolated by streptavidin coated magnetic particles.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 BloodHome page
D. J. Hussey, M. Nicola, S. Moore, G. B. Peters, and A. Dobrovic
The (4;11)(q21;p15) Translocation Fuses the NUP98 and RAP1GDS1 Genes and Is Recurrent in T-Cell Acute Lymphocytic Leukemia
Blood, September 15, 1999; 94(6): 2072 - 2079.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
S. Lankiewicz, N. Lobitz, C. H. R. Wetzel, R. Rupprecht, G. Gisselmann, and H. Hatt
Molecular Cloning, Functional Expression, and Pharmacological Characterization of 5-Hydroxytryptamine3 Receptor cDNA and Its Splice Variants from Guinea Pig
Mol. Pharmacol., February 1, 1998; 53(2): 202 - 212.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.