Nucleic Acids Research, Vol 25, Issue 11 2098-2105, Copyright © 1997 by Oxford University Press
D Mumberg, M Wick, C Burger, K Haas, M Funk and R Muller
Cyclin E is the regulatory subunit of the cdc2-related protein kinase cdk2
and is a rate limiting factor for the entry into S phase. To date, cyclin E
is the only cyclin for which alternative splicing has been described. We
report here the isolation of a new splice variant of cyclin E, termed
cyclin ET, which has an internal deletion of 45 amino acids compared with
the full-length cyclin E protein. Even though cyclin ETcontains an intact
cyclin box, it is unable to complement a triple cln mutant strain of
Saccharomyces cerevisiae or to interfere with rescue by cyclin E,
indicating that an intact cyclin box is functionally insufficient. The
expression pattern of cyclin ET during cell cycle entry, progression and
differentiation differs from that of cyclin E. Thus, ET expression precedes
that of the other isoforms during the G0-->S progression; it shows a
sharp peak in early G1 in cells released from a mitotic block and is
strongly down-regulated in terminally differentiated myeloid cells. These
observations point to different functions for cyclin ET and E and show for
the first time that the alternative splicing of cyclin E is a regulated
mechanism governed by the cell cycle and differentiation.
ARTICLES
Cyclin ET, a new splice variant of human cyclin E with a unique expression pattern during cell cycle progression and differentiation
Institut fur Molekularbiologie und Tumorforschung (IMT), Philipps- Universitat Marburg, Emil-Mannkopff-Strasse 2, D-35033 Marburg, Germany.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. M. Sieuwerts, M. P. Look, M. E. Meijer-van Gelder, M. Timmermans, A. M.A.C. Trapman, R. R. Garcia, M. Arnold, A. J.W. Goedheer, V. de Weerd, H. Portengen, et al. Which cyclin e prevails as prognostic marker for breast cancer? Results from a retrospective study involving 635 lymph node-negative breast cancer patients. Clin. Cancer Res., June 1, 2006; 12(11): 3319 - 3328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Lu, K. A. Seta, and D. E. Millhorn Novel Role for Cyclin-dependent Kinase 2 in Neuregulin-induced Acetylcholine Receptor {epsilon} Subunit Expression in Differentiated Myotubes J. Biol. Chem., June 10, 2005; 280(23): 21731 - 21738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Geisen and T. Moroy The Oncogenic Activity of Cyclin E Is Not Confined to Cdk2 Activation Alone but Relies on Several Other, Distinct Functions of the Protein J. Biol. Chem., October 11, 2002; 277(42): 39909 - 39918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bukovsky, M. Cekanova, M. R. Caudle, J. Wimalasena, J. S. Foster, J. A. Keenan, and R. F. Elder Variability of Placental Expression of Cyclin E Low Molecular Weight Variants Biol Reprod, August 1, 2002; 67(2): 568 - 574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Vandepoele, J. Raes, L. De Veylder, P. Rouze, S. Rombauts, and D. Inze Genome-Wide Analysis of Core Cell Cycle Genes in Arabidopsis PLANT CELL, April 1, 2002; 14(4): 903 - 916. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gao and D. R. Richardson The potential of iron chelators of the pyridoxal isonicotinoyl hydrazone class as effective antiproliferative agents, IV: the mechanisms involved in inhibiting cell-cycle progression Blood, August 1, 2001; 98(3): 842 - 850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Porter and K. Keyomarsi Novel splice variants of cyclin E with altered substrate specificity Nucleic Acids Res., December 1, 2000; 28(23): e101 - e101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Pestell, C. Albanese, A. T. Reutens, J. E. Segall, R. J. Lee, and A. Arnold The Cyclins and Cyclin-Dependent Kinase Inhibitors in Hormonal Regulation of Proliferation and Differentiation Endocr. Rev., August 1, 1999; 20(4): 501 - 534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. DONNELLAN and R. CHETTY Cyclin E in human cancers FASEB J, May 1, 1999; 13(8): 773 - 780. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. M. Gudas, M. Payton, S. Thukral, E. Chen, M. Bass, M. O. Robinson, and S. Coats Cyclin E2, a Novel G1 Cyclin That Binds Cdk2 and Is Aberrantly Expressed in Human Cancers Mol. Cell. Biol., January 1, 1999; 19(1): 612 - 622. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Peng, Y. Zhu, J. T. Milton, and D. H. Price Identification of multiple cyclin subunits of human P-TEFb Genes & Dev., March 1, 1998; 12(5): 755 - 762. [Abstract] [Full Text]
|
|
|
|
 |
|
 J.-C. Lozano, P. Schatt, F. Marques, G. Peaucellier, P. Fort, J.-P. Feral, A.-M. Geneviere, and A. Picard A Presumptive Developmental Role for a Sea Urchin Cyclin B Splice Variant J. Cell Biol., January 26, 1998; 140(2): 283 - 293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, F. Liu, and M. L. Adamo Cyclic AMP Inhibits Extracellular Signal-regulated Kinase and Phosphatidylinositol 3-Kinase/Akt Pathways by Inhibiting Rap1 J. Biol. Chem., September 28, 2001; 276(40): 37242 - 37249. [Abstract] [Full Text] [PDF]
|
|