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Nucleic Acids Research, Vol 25, Issue 12 2301-2302, Copyright © 1997 by Oxford University Press

ARTICLES

BstAPI, an ApaBI isoschizomer, cleaves DNA at 5'-GCANNNN NTGC-3'

MA Abdurashitov, OA Belichenko, AV Shevchenko, VS Dedkov and SK Degtyarev
SibEnzyme, 8 Lavrentiev Str., Novosibirsk 630090, Russia. info@siben.nsk.su

Cleavage positions of Bst API, a new restriction endonuclease (ENase) that recognizes palindromic interrupted DNA sequence, have been determined. Recognition sequences and cleavage sites comparison shows that Bst API shares similarity with a number of type II restriction enzymes.
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S. Kh. Degtyarev, O. A. Belichenko, N. A. Lebedeva, V. S. Dedkov, and M. A. Abdurashitov
BtrI, a novel restriction endonuclease, recognises the non-palindromic sequence 5'-CACGTC(-3/-3)-3'
Nucleic Acids Res., June 1, 2000; 28(11): e56 - e56.
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