Nucleic Acids Research, Vol 25, Issue 12 2319-2325, Copyright © 1997 by Oxford University Press
A Weihe, B Hedtke and T Borner
We have cloned a full-length cDNA from the higher plant Chenopodium album
coding for a single subunit bacteriophage-type RNA polymerase. The cDNA
isolated from an actively growing cell suspension culture recognized a 3.8
kb transcript on Northern blots. The open reading frame comprises 987 amino
acids with a predicted molecular mass of 112 kDa. A comparison of the
protein sequence with those of the two known fungal mitochondrial RNA
polymerases, from Saccharomyces cerevisiae and Neurospora crassa , reveals
extensive homology between the three enzymes. with complete conservation of
all catalytically essential amino acids. The putative mitochondrial RNA
polymerase from C.album , as well as homologous sequences from rice and
barley, which have been partially cloned, lack two catalytically
non-essential regions of up to 176 amino acids near the C-terminus present
in the two fungal mitochondrial RNA polymerases. The extreme N-terminus of
the cloned C.album RNA polymerase displays features of a potential
mitochondrial transit sequence. In phylogenetic trees constructed to
compare the evolutionary relationships between the different single subunit
RNA polymerases the C.album sequence forms a subgroup together with the
S.cerevisiae and the N.crassa mitochondrial RNA polymerases, well
separating from both bacteriophage enzymes and plasmid-encoded RNA
polymerases found in mitochondria of many fungi and some higher plants.
ARTICLES
Cloning and characterization of a cDNA encoding a bacteriophage-type RNA polymerase from the higher plant Chenopodium album
Institute of Biology, Humboldt University Berlin, Chausseestrasse 117, D-10115 Berlin, Germany. andreas=weihe@biologire.hu-berlin.de
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