Nucleic Acids Research, Vol 25, Issue 12 2509-2515, Copyright © 1997 by Oxford University Press
TL Bean and PA Ney
Regulated expression of genes in the beta-globin cluster depends upon
sequences located between 5 and 20 kb upstream of the epsilon gene, known
as the locus control region (LCR). beta-Globin expression in murine
erythroleukemia (MEL) cells depends on NF-E2, a transcription factor which
binds to enhancer sequences in the LCR. To gain insight into the mechanism
of globin gene activation by NF-E2, an NF-E2 null MEL cell line was used to
map regions of NF-E2 required for beta-globin expression. Within the
transactivation domain, two discrete proline- rich regions were required
for rescue of beta-globin expression. The first was located at the
N-terminus of NF-E2, while the second was located N-terminal of the cap 'n
collar (CNC) domain. Other proline- rich sequences were dispensable,
indicating that proline content per se does not determine NF-E2 activity.
Mutations within the conserved CNC domain markedly diminished rescue of
beta-globin expression. This domain was required, in addition to the basic
leucine zipper domain, for DNA binding activity. The requirement for
discrete proline-rich sequences within the transactivation domain suggests
that globin gene expression in MEL cells depends on specific interactions
between NF-E2 and downstream effector molecules.
ARTICLES
Multiple regions of p45 NF-E2 are required for beta-globin gene expression in erythroid cells
Department of Biochemistry, Saint Jude Children's Research Hospital, 332 North Lauderdale, Memphis, TN 38101, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y.-C. Shyu, T.-L. Lee, C.-Y. Ting, S.-C. Wen, L.-J. Hsieh, Y.-C. Li, J.-l. Hwang, C.-C. Lin, and C.-K. J. Shen Sumoylation of p45/NF-E2: Nuclear Positioning and Transcriptional Activation of the Mammalian {beta}-Like Globin Gene Locus Mol. Cell. Biol., December 1, 2005; 25(23): 10365 - 10378. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Kiekhaefer, M. E. Boyer, K. D. Johnson, and E. H. Bresnick A WW Domain-binding Motif within the Activation Domain of the Hematopoietic Transcription Factor NF-E2 Is Essential for Establishment of a Tissue-specific Histone Modification Pattern J. Biol. Chem., February 27, 2004; 279(9): 7456 - 7461. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Loyd, Y. Okamoto, M. S. Randall, and P. A. Ney Role of AP1/NFE2 binding sites in endogenous {alpha}-globin gene transcription Blood, December 1, 2003; 102(12): 4223 - 4228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Dai, S. Petruccelli, M. I. Ordiz, Z. Zhang, S. Chen, and R. N. Beachy Functional Analysis of RF2a, a Rice Transcription Factor J. Biol. Chem., September 19, 2003; 278(38): 36396 - 36402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Kiekhaefer, J. A. Grass, K. D. Johnson, M. E. Boyer, and E. H. Bresnick Hematopoietic-specific activators establish an overlapping pattern of histone acetylation and methylation within a mammalian chromatin domain PNAS, October 29, 2002; 99(22): 14309 - 14314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. D. Johnson, J. A. Grass, M. E. Boyer, C. M. Kiekhaefer, G. A. Blobel, M. J. Weiss, and E. H. Bresnick Cooperative activities of hematopoietic regulators recruit RNA polymerase II to a tissue-specific chromatin domain PNAS, September 3, 2002; 99(18): 11760 - 11765. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. D. Johnson, J. E. Norton, and E. H. Bresnick Requirements for utilization of CREB binding protein by hypersensitive site two of the {beta}-globin locus control region Nucleic Acids Res., April 1, 2002; 30(7): 1522 - 1530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Brown, S. Pattison, J. van Ree, E. Coghill, A. Perkins, S. M. Jane, and J. M. Cunningham Distinct Domains of Erythroid Kruppel-Like Factor Modulate Chromatin Remodeling and Transactivation at the Endogenous {beta}-Globin Gene Promoter Mol. Cell. Biol., January 1, 2002; 22(1): 161 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Persons, E. R. Allay, D. E. Sabatino, P. Kelly, D. M. Bodine, and A. W. Nienhuis Functional requirements for phenotypic correction of murine {beta}-thalassemia: implications for human gene therapy Blood, May 15, 2001; 97(10): 3275 - 3282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Veraksa, N McGinnis, X Li, J Mohler, and W McGinnis Cap 'n' collar B cooperates with a small Maf subunit to specify pharyngeal development and suppress deformed homeotic function in the Drosophila head Development, January 9, 2000; 127(18): 4023 - 4037. [Abstract] [PDF]
|
|
|
|
|
|
E. C. Forsberg, K. Johnson, T. N. Zaboikina, E. A. Mosser, and E. H. Bresnick Requirement of an E1A-sensitive Coactivator for Long-range Transactivation by the beta -Globin Locus Control Region J. Biol. Chem., September 17, 1999; 274(38): 26850 - 26859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. K. Versaw, V. Blank, N. M. Andrews, and E. H. Bresnick Mitogen-activated protein kinases enhance long-range activation by the beta -globin locus control region PNAS, July 21, 1998; 95(15): 8756 - 8760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Casteel, M. Suhasini, T. Gudi, R. Naima, and R. B. Pilz Regulation of the Erythroid Transcription Factor NF-E2 by Cyclic Adenosine Monophosphate-Dependent Protein Kinase Blood, May 1, 1998; 91(9): 3193 - 3201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Martin, J. M. van Deursen, R. A. Shivdasani, C. W. Jackson, A. G. Troutman, and P. A. Ney Erythroid Maturation and Globin Gene Expression in Mice With Combined Deficiency of NF-E2 and Nrf-2 Blood, May 1, 1998; 91(9): 3459 - 3466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-L. Hung, A. Y. Kim, W. Hong, C. Rakowski, and G. A. Blobel Stimulation of NF-E2 DNA Binding by CREB-binding Protein (CBP)-mediated Acetylation J. Biol. Chem., March 30, 2001; 276(14): 10715 - 10721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Husberg, P. Murphy, E. Martin, and A.-B. Kolsto Two Domains of the Human bZIP Transcription Factor TCF11 Are Necessary for Transactivation J. Biol. Chem., May 18, 2001; 276(21): 17641 - 17652. [Abstract] [Full Text] [PDF]
|
|