Nucleic Acids Research, Vol 25, Issue 13 2620-2626, Copyright © 1997 by Oxford University Press
LE Bird, K Hakansson, H Pan and DB Wigley
Limited proteolysis of bacteriophage T7 primase/helicase with
endoproteinase Glu-C produces several proteolytic fragments. One of these
fragments, which is derived from the C-terminal region of the protein, was
prepared and shown to retain helicase activity. This result supports a
model in which the gene 4 proteins consist of functionally separable
domains. Crystals of this C-terminal fragment of the protein have been
obtained that are suitable for X-ray diffraction studies.
ARTICLES
Characterization and crystallization of the helicase domain of bacteriophage T7 gene 4 protein
Laboratory of Molecular Biophysics, Rex Richards Building, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
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