Nucleic Acids Research, Vol 25, Issue 13 2681-2682, Copyright © 1997 by Oxford University Press
MJ O'Neill and AH Sinclair
Representational difference analysis (RDA) is a powerful technique for
cloning the differences between genomes, and has recently been adapted for
cloning differentially expressed genes. RDA, like other PCR-based
differential screening methods, is prone to the production of false
positives. We have identified a major source of false positives in RDA of
cDNA and have introduced improvements which minimise their production.
These modifications also significantly increase sensitivity, allowing for
the isolation of rare differential transcripts from nanogram amounts of
mRNA.
ARTICLES
Isolation of rare transcripts by representational difference analysis
Department of Paediatrics and Centre for Hormone Research, The University of Melbourne, Royal Children's Hospital, Melbourne, Victoria 3052, Australia. oneill@cryptic.rch.unimelb.edu.au
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