Nucleic Acids Research, Vol 25, Issue 14 2723-2729, Copyright © 1997 by Oxford University Press
U Baron, M Gossen and H Bujard
Several tetracycline-controlled transactivators (tTA) were generated which
differ in their activation potential by >3 orders of magnitude. The
transactivators are fusions between the Tet repressor and minimal
transcriptional activation domains derived from Herpes simplex virus
protein 16 (VP16). By reducing the VP16 moiety of the previously described
tTA to 12 amino acids, potential targets for interactions with various
cellular transcription factors were eliminated, as were potential epitopes
which may elicit a cellular immune response. When compared with the
originally described tTA, these new transactivators are tolerated at higher
intracellular concentrations. This will facilitate establishment of tet
regulatory systems under a variety of conditions, but particularly when
cell type-restricted tetracycline- controlled gene expression is to be
achieved in transgenic organisms via homologous recombination.
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Tetracycline-controlled transcription in eukaryotes: novel transactivators with graded transactivation potential
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