Nucleic Acids Research, Vol 25, Issue 14 2902-2910, Copyright © 1997 by Oxford University Press
MA Lochrie, S Waugh, DG Pratt Jr, J Clever, TG Parslow and B Polisky
RNA ligands that bind to the human immunodeficiency virus type-1 (HIV- 1)
gag polyprotein with 10(-9) M affinity were isolated from a complex pool of
RNAs using an in vitro selection method. The ligands bind to two different
regions within gag, either to the matrix protein or to the nucleocapsid
protein. Binding of a matrix ligand to gag did not interfere with the
binding of a nucleocapsid ligand, and binding of a nucleocapsid ligand to
gag did not interfere with the binding of a matrix ligand. However, binding
of a nucleocapsid ligand to gag did interfere with binding of an RNA
containing the HIV-1 RNA packaging element (psi), even though the sequence
of the nucleocapsid ligand is not similar topsi. The minimal sequences
required for the ligands to bind to matrix or nucleocapsid were determined.
Minimal nucleocapsid ligands are predicted to form a stem-loop structure
that has a self- complementary sequence at one end. Minimal matrix ligands
are predicted to form a different stem-loop structure that has a CAARU loop
sequence. The properties of these RNA ligands may provide tools for
studying RNA interactions with matrix and nucleocapsid, and a novel method
for inhibiting HIV replication.
ARTICLES
In vitro selection of RNAs that bind to the human immunodeficiency virus type-1 gag polyprotein
NeXstar Pharmaceuticals, Inc., 2860 Wilderness Place, Boulder, CO 80301, USA. mlochrie@nexstar.com
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. I. Jang, Y. H. Kim, S. Y. Paik, and J. C. You Development of a Cell-Based Assay Probing the Specific Interaction between the Human Immunodeficiency Virus Type 1 Nucleocapsid and Psi RNA In Vivo J. Virol., June 1, 2007; 81(11): 6151 - 6155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. James Aptamers in the virologists' toolkit J. Gen. Virol., February 1, 2007; 88(2): 351 - 364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Ott, L. V. Coren, and T. D. Gagliardi Redundant Roles for Nucleocapsid and Matrix RNA-Binding Sequences in Human Immunodeficiency Virus Type 1 Assembly J. Virol., November 15, 2005; 79(22): 13839 - 13847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Muriaux, S. Costes, K. Nagashima, J. Mirro, E. Cho, S. Lockett, and A. Rein Role of Murine Leukemia Virus Nucleocapsid Protein in Virus Assembly J. Virol., November 15, 2004; 78(22): 12378 - 12385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. LANCHY, J. D. IVANOVITCH, and J. S. LODMELL A structural linkage between the dimerization and encapsidation signals in HIV-2 leader RNA RNA, August 1, 2003; 9(8): 1007 - 1018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Kemler, R. Barraza, and E. M. Poeschla Mapping the Encapsidation Determinants of Feline Immunodeficiency Virus J. Virol., October 25, 2002; 76(23): 11889 - 11903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Clever, D. Miranda Jr., and T. G. Parslow RNA Structure and Packaging Signals in the 5' Leader Region of the Human Immunodeficiency Virus Type 1 Genome J. Virol., October 25, 2002; 76(23): 12381 - 12387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Yu, S. M. Joshi, Y. M. Ma, R. L. Kingston, M. N. Simon, and V. M. Vogt Characterization of Rous Sarcoma Virus Gag Particles Assembled In Vitro J. Virol., March 15, 2001; 75(6): 2753 - 2764. [Abstract] [Full Text]
|
|
|
|
|
|
N. A. Jewell and L. M. Mansky In the beginning: genome recognition, RNA encapsidation and the initiation of complex retrovirus assembly J. Gen. Virol., August 1, 2000; 81(8): 1889 - 1899. [Full Text]
|
|
|
|
|
|
J. L. Clever, R. A. Taplitz, M. A. Lochrie, B. Polisky, and T. G. Parslow A Heterologous, High-Affinity RNA Ligand for Human Immunodeficiency Virus Gag Protein Has RNA Packaging Activity J. Virol., January 1, 2000; 74(1): 541 - 546. [Abstract] [Full Text]
|
|
|
|
|
|
M. T. Burniston, A. Cimarelli, J. Colgan, S. P. Curtis, and J. Luban Human Immunodeficiency Virus Type 1 Gag Polyprotein Multimerization Requires the Nucleocapsid Domain and RNA and Is Promoted by the Capsid-Dimer Interface and the Basic Region of Matrix Protein J. Virol., October 1, 1999; 73(10): 8527 - 8540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Jayasena Aptamers: An Emerging Class of Molecules That Rival Antibodies in Diagnostics Clin. Chem., September 1, 1999; 45(9): 1628 - 1650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Helga-Maria, M.-L. Hammarskjöld, and D. Rekosh An Intact TAR Element and Cytoplasmic Localization Are Necessary for Efficient Packaging of Human Immunodeficiency Virus Type 1 Genomic RNA J. Virol., May 1, 1999; 73(5): 4127 - 4135. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Bukrinskaya, B. Brichacek, A. Mann, and M. Stevenson Establishment of a Functional Human Immunodeficiency Virus Type 1 (HIV-1) Reverse Transcription Complex Involves the Cytoskeleton J. Exp. Med., December 7, 1998; 188(11): 2113 - 2125. [Abstract] [Full Text] [PDF]
|
|