Nucleic Acids Research, Vol 25, Issue 15 2973-2978, Copyright © 1997 by Oxford University Press
N Suzuki, A Shimamoto, O Imamura, J Kuromitsu, S Kitao, M Goto and Y Furuichi
The gene responsible for Werner's syndrome (WRN) contains a region
homologous to the Escherichia coli RecQ type DNA helicase and was thought
to code for a DNA helicase belonging to this helicase family. However, no
evidence has been shown before to substantiate this prediction. Here, we
show data that the product of the WRN gene is indeed a DNA helicase. The
gene product, a polypeptide with a relative molecular mass of 170 kDa,
expressed in the insect Spodoptera frugiperda (Sf21) cell and purified by
affinity column chromatography contained both the ATPase and DNA unwinding
activities characteristic of DNA helicase. Expressions in Sf21, as well as
in HeLa cells, showed that the WRN DNA helicase is exclusively transported
to the nucleoplasm, which is consistent with its function in DNA
metabolism. Our studies on strand displacement suggest that WRN helicase
can unwind not only a duplex DNA, but also an RNA-DNA heteroduplex, while
the latter reaction seems less efficient. Enzymological features learned
from the purified WRN helicase are discussed with respect to the biological
function, which remains to be clarified.
ARTICLES
DNA helicase activity in Werner's syndrome gene product synthesized in a baculovirus system
AGENE Research Institute, 200 Kajiwara, Kamakura, Kanagawa 247, Japan.
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Y.-i. Kawabe, M. Seki, T. Seki, W.-S. Wang, O. Imamura, Y. Furuichi, H. Saitoh, and T. Enomoto Covalent Modification of the Werner's Syndrome Gene Product with the Ubiquitin-related Protein, SUMO-1 J. Biol. Chem., July 7, 2000; 275(28): 20963 - 20966. [Abstract] [Full Text] [PDF]
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B. Li and L. Comai Functional Interaction between Ku and the Werner Syndrome Protein in DNA End Processing J. Biol. Chem., September 8, 2000; 275(37): 28349 - 28352. [Abstract] [Full Text] [PDF]
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