Nucleic Acids Research, Vol 25, Issue 15 2992-3000, Copyright © 1997 by Oxford University Press
MC Huber, U Jagle, G Kruger and C Bonifer
The complete chicken lysozyme locus is expressed in a position independent
fashion in macrophages of transgenic mice and forms the identical chromatin
structure as observed with the endogenous gene in chicken cells. Individual
lysozyme cis -regulatory elements reorganize their chromatin structure at
different developmental stages. Accordingly, their activities are
developmentally regulated, indicating a differential role of these elements
in locus activation. We have shown previously that a subset of enhancer
elements and the promoter are sufficient to activate transcription of the
chicken lysozyme gene at the correct developmental stage. Here, we analyzed
to which grade the developmentally controlled chromatin reorganizing
capacity of cis - regulatory elements in the 5'-region of the chicken
lysozyme locus is dependent on promoter elements, and we examined whether
the lysozyme locus carries a dominant chromatin reorganizing element. To
this end we generated transgenic mouse lines carrying constructs with a
deletion of the lysozyme promoter. Expression of the transgene in
macrophages is abolished, however, the chromatin reorganizing ability of
the cis - regulatory elements is differentially impaired. Some cis
-elements require the interaction with the promoter to stabilize
transcription factor complexes detectable as DNase I hypersensitive sites
in chromatin, whereas other elements reorganize their chromatin structure
autonomously.
ARTICLES
The developmental activation of the chicken lysozyme locus in transgenic mice requires the interaction of a subset of enhancer elements with the promoter
Institut fur Biologie III der Universitat Freiburg, Schanzlestrasse 1, D-79104 Freiburg, Germany.
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