Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (228K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (23)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Fahy, E.
Right arrow Articles by Ghosh, S. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fahy, E.
Right arrow Articles by Ghosh, S. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 25, Issue 15 3102-3109, Copyright © 1997 by Oxford University Press

ARTICLES

Multiplex fluorescence-based primer extension method for quantitative mutation analysis of mitochondrial DNA and its diagnostic application for Alzheimer's disease

E Fahy, R Nazarbaghi, M Zomorrodi, C Herrnstadt, WD Parker, RE Davis and SS Ghosh
MitoKor, 11494 Sorrento Valley Road, San Diego, CA 92121, USA.

A sensitive and highly reproducible multiplexed primer extension assay is described for quantitative mutation analysis of heterogeneous DNA populations. Wild-type and mutant target DNA are simultaneously probed in competitive primer extension reactions using fluorophor-labeled primers and high fidelity, thermostable DNA polymerases in the presence of defined mixtures of deoxy- and dideoxynucleotides. Primers are differentially extended and the resulting products are distinguished by size and dye label. Wild-type:mutant DNA ratios are determined from the fluorescence intensities associated with electrophoretically resolved reaction products. Multiple nucleotide sites can be simultaneously interrogated with uniquely labeled primers of different lengths. The application of this quantitative technique is shown in the analysis of heteroplasmic point mutations in mitochondrial DNA that are associated with Alzheimer's disease.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 IOVSHome page
K. K. Abu-Amero, J. Morales, M. N. Osman, and T. M. Bosley
Nuclear and Mitochondrial Analysis of Patients with Primary Angle-Closure Glaucoma
Invest. Ophthalmol. Vis. Sci., December 1, 2007; 48(12): 5591 - 5596.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
J. L. Garcia-Perez, A. J. Doucet, A. Bucheton, J. V. Moran, and N. Gilbert
Distinct mechanisms for trans-mediated mobilization of cellular RNAs by the LINE-1 reverse transcriptase
Genome Res., May 1, 2007; 17(5): 602 - 611.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. K. Abu-Amero and T. M. Bosley
Mitochondrial Abnormalities in Patients with LHON-like Optic Neuropathies.
Invest. Ophthalmol. Vis. Sci., October 1, 2006; 47(10): 4211 - 4220.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. K. Abu-Amero, J. Morales, and T. M. Bosley
Mitochondrial abnormalities in patients with primary open-angle glaucoma.
Invest. Ophthalmol. Vis. Sci., June 1, 2006; 47(6): 2533 - 2541.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Karadag, M. Riminucci, P. Bianco, N. Cherman, S. A. Kuznetsov, N. Nguyen, M. T. Collins, P. G. Robey, and L. W. Fisher
A novel technique based on a PNA hybridization probe and FRET principle for quantification of mutant genotype in fibrous dysplasia/McCune-Albright syndrome
Nucleic Acids Res., April 19, 2004; 32(7): e63 - e63.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
P. E. Bennett-Baker, J. Wilkowski, and D. T. Burke
Age-Associated Activation of Epigenetically Repressed Genes in the Mouse
Genetics, December 1, 2003; 165(4): 2055 - 2062.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. K. Tong and J. Ju
Single nucleotide polymorphism detection by combinatorial fluorescence energy transfer tags and biotinylated dideoxynucleotides
Nucleic Acids Res., March 1, 2002; 30(5): e19 - e19.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
P. F. Chinnery, D. T. Brown, R. M. Andrews, R. Singh-Kler, P. Riordan-Eva, J. Lindley, D. A. Applegarth, D. M. Turnbull, and N. Howell
The mitochondrial ND6 gene is a hot spot for mutations that cause Leber's hereditary optic neuropathy
Brain, January 1, 2001; 124(1): 209 - 218.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Hirano, A. Shtilbans, R. Mayeux, M. M. Davidson, S. DiMauro, J. A. Knowles, and E. A. Schon
Apparent mtDNA heteroplasmy in Alzheimer's disease patients and in normals due to PCR amplification of nucleus-embedded mtDNA pseudogenes
PNAS, December 23, 1997; 94(26): 14894 - 14899.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.