Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (65K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (23)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Shepard, A. R.
Right arrow Articles by Rae, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shepard, A. R.
Right arrow Articles by Rae, J. L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 25, Issue 15 3183-3185, Copyright © 1997 by Oxford University Press

ARTICLES

Magnetic bead capture of cDNAs from double-stranded plasmid cDNA libraries

AR Shepard and JL Rae
Departments of Physiology/Biophysics and Ophthalmology, Mayo Foundation, 200 1st Street SW, Rochester, MN 55905, USA.

We have developed a cDNA library screening method which allows the simultaneous screening of >10 ( 12 ) double-stranded plasmid cDNA molecules with minimal a priori sequence knowledge. A biotinylated, gene-specific oligonucleotide probe along with abutting 'blocking' oligos is hybridized to the plasmid cDNA library and the target plasmid retrieved with paramagnetic streptavidin beads and transformed into Escherichia coli. Multiple rounds of enrichment with a target plasmid represented at 0.002-0.0001% resulted in over one-third positive clones. Our method will be useful for isolating even the rarest cDNAs starting from ESTs, isolated exons or homologous sequence information.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GeneticsHome page
E. Hamilton, P. Bruns, C. Lin, V. Merriam, E. Orias, L. Vong, and D. Cassidy-Hanley
Genome-Wide Characterization of Tetrahymena thermophila Chromosome Breakage Sites. I. Cloning and Identification of Functional Sites
Genetics, August 1, 2005; 170(4): 1611 - 1621.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
G. L. Lyford, P. R. Strege, A. Shepard, Y. Ou, L. Ermilov, S. M. Miller, S. J. Gibbons, J. L. Rae, J. H. Szurszewski, and G. Farrugia
alpha 1C (CaV1.2) L-type calcium channel mediates mechanosensitive calcium regulation
Am J Physiol Cell Physiol, September 1, 2002; 283(3): C1001 - C1008.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
L. D. Bowler, M. Hubank, and B. G. Spratt
Representational difference analysis of cDNA for the detection of differential gene expression in bacteria: development using a model of iron-regulated gene expression in Neisseria meningitidis
Microbiology, December 1, 1999; 145(12): 3529 - 3537.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. R. Shepard and J. L. Rae
Electrically silent potassium channel subunits from human lens epithelium
Am J Physiol Cell Physiol, September 1, 1999; 277(3): C412 - C424.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. A. Chavez, A. T. Gray, B. B. Zhao, C. H. Kindler, M. J. Mazurek, Y. Mehta, J. R. Forsayeth, and C. S. Yost
TWIK-2, a New Weak Inward Rectifying Member of the Tandem Pore Domain Potassium Channel Family
J. Biol. Chem., March 19, 1999; 274(12): 7887 - 7892.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. O. Bukanov, V. V. Demidov, P. E. Nielsen, and M. D. Frank-Kamenetskii
PD-loop: A complex of duplex DNA with an oligonucleotide
PNAS, May 12, 1998; 95(10): 5516 - 5520.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. I. Muir, L. Chamberlain, N. A. Elshourbagy, D. Michalovich, D. J. Moore, A. Calamari, P. G. Szekeres, H. M. Sarau, J. K. Chambers, P. Murdock, et al.
AXOR12, a Novel Human G Protein-coupled Receptor, Activated by the Peptide KiSS-1
J. Biol. Chem., July 27, 2001; 276(31): 28969 - 28975.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.