Nucleic Acids Research, Vol 25, Issue 16 3281-3289, Copyright © 1997 by Oxford University Press
LM Brown and DS Ray
Transcripts of both mitochondrial and nuclear DNA replication genes
accumulate periodically during the cell cycle in Crithidia fasciculata. An
octameric consensus sequence with a conserved hexameric core was found
previously to be required for cycling of the TOP2 transcript, encoding the
mitochondrial DNA topoisomerase. We show here that the rate of synthesis of
the p51 protein, the large subunit of nuclear replication protein-A encoded
by the RPA1 gene, varies during the cell cycle in parallel with RPA1 mRNA
level. Plasmids expressing a truncated form of RPA1 (Delta RPA1 ) were used
to identify cis elements required for cycling of the Delta RPA1 transcript.
Sequences within the RPA1 5'- untranslated region (UTR) were found to be
necessary for cycling of the Delta RPA1 transcript. These sequences also
function when transposed 3'of the Delta RPA1 coding sequence. A 121 bp
fragment of this sequence can confer cycling on a heterologous transcript,
but is inactivated when two consensus octamers within the sequence are
mutated. Mutation of these two octamers in the full-length 5'-UTR ofDelta
RPA1 is insufficient to abolish cycling of the mRNA unless three additional
octamers having single base changes within the hexameric core are also
mutated. Thus, common octameric sequence elements are involved in periodic
accumulation of both the TOP2 and RPA1 transcripts.
ARTICLES
Cell cycle regulation of RPA1 transcript levels in the trypanosomatid Crithidia fasciculata
Molecular Biology Institute and Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095- 1570, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Li, Y. Sun, J. C. Hines, and D. S. Ray Identification of New Kinetoplast DNA Replication Proteins in Trypanosomatids Based on Predicted S-Phase Expression and Mitochondrial Targeting Eukaryot. Cell, December 1, 2007; 6(12): 2303 - 2310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Sinha, J. C. Hines, and D. S. Ray Cell Cycle-Dependent Localization and Properties of a Second Mitochondrial DNA Ligase in Crithidia fasciculata Eukaryot. Cell, January 1, 2006; 5(1): 54 - 61. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Rider Jr., X. Cai, W. J. Sullivan Jr., A. T. Smith, J. Radke, M. White, and G. Zhu The Protozoan Parasite Cryptosporidium parvum Possesses Two Functionally and Evolutionarily Divergent Replication Protein A Large Subunits J. Biol. Chem., September 9, 2005; 280(36): 31460 - 31469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. WEBB, R. BURNS, N. KIMBLIN, L. ELLIS, and M. CARRINGTON A novel strategy to identify the location of necessary and sufficient cis-acting regulatory mRNA elements in trypanosomes RNA, July 1, 2005; 11(7): 1108 - 1116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Mittra and D. S. Ray Presence of a Poly(A) Binding Protein and Two Proteins with Cell Cycle-Dependent Phosphorylation in Crithidia fasciculata mRNA Cycling Sequence Binding Protein II Eukaryot. Cell, October 1, 2004; 3(5): 1185 - 1197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Millership, X. Cai, and G. Zhu Functional characterization of replication protein A2 (RPA2) from Cryptosporidium parvum Microbiology, May 1, 2004; 150(5): 1197 - 1205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. K. Avliyakulov, J. C. Hines, and D. S. Ray Sequence Elements in both the Intergenic Space and the 3' Untranslated Region of the Crithidia fasciculata KAP3 Gene Are Required for Cell Cycle Regulation of KAP3 mRNA Eukaryot. Cell, August 1, 2003; 2(4): 671 - 677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Mittra, K. M. Sinha, J. C. Hines, and D. S. Ray Presence of Multiple mRNA Cycling Sequence Element-binding Proteins in Crithidia fasciculata J. Biol. Chem., July 11, 2003; 278(29): 26564 - 26571. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Mahmood, B. Mittra, J. C. Hines, and D. S. Ray Characterization of the Crithidia fasciculata mRNA Cycling Sequence Binding Proteins Mol. Cell. Biol., July 15, 2001; 21(14): 4453 - 4459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mahmood, J. C. Hines, and D. S. Ray Identification of cis and trans Elements Involved in the Cell Cycle Regulation of Multiple Genes in Crithidia fasciculata Mol. Cell. Biol., September 1, 1999; 19(9): 6174 - 6182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Johnson and P. T. Englund Changes in Organization of Crithidia fasciculata Kinetoplast DNA Replication Proteins during the Cell Cycle J. Cell Biol., November 16, 1998; 143(4): 911 - 919. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mahmood and D. S. Ray Nulear Extracts of Crithidia fasciculata Contain a Factor(s) That Binds to the 5'-Untranslated Regions of TOP2 and RPA1 mRNAs Containing Sequences Required for Their Cell Cycle Regulation J. Biol. Chem., September 11, 1998; 273(37): 23729 - 23734. [Abstract] [Full Text] [PDF]
|
|