Nucleic Acids Research, Vol 25, Issue 17 3415-3420, Copyright © 1997 by Oxford University Press
S Oda, E Oki, Y Maehara and K Sugimachi
The instability of microsatellite sequences dispersed in the genome has
been linked to a deficiency in cellular mismatch repair. This phenotype has
been frequently observed in various human neoplasms and is regarded as a
major factor in tumorigenesis. To demonstrate alterations in microsatellite
sequences, polymerase chain reaction (PCR) and electrophoretic analysis are
techniques often used. However, the electrophoretic profiles of
PCR-amplified microsatellite sequences have not been well characterized.
Moreover, the conventional method using autoradiography has critical
problems in detection characteristics and migration accuracy. We made use
of fluorescence-labeled PCR and laser scanning with linear detection
characteristics, so as to detect bands quantitatively. Next, we
characterized Taq polymerase-dependent modification of the amplified
microsatellite sequences, using artificially synthesized microsatellite
alleles and we optimized the electrophoretic profiles by enzymatic
modification with T4 DNA polymerase. We developed a dual fluorescence
co-electrophoresis system, in which both samples derived from cancer and
normal tissues are electrophoresed in the same lane, in order to minimize
migration errors. These improvements remarkably facilitate precise and
objective assessments of microsatellite instability. Analyzing many
positive cases in cell lines and tissue specimens, we classified all the
patterns of microsatellite alteration and set up new criteria for assessing
microsatellite instability.
ARTICLES
Precise assessment of microsatellite instability using high resolution fluorescent microsatellite analysis
Cancer Center, Kyushu University Hospital, Fukuoka 812-82, Japan. shinya@cancer.med.kyushu-u.ac.jp
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. L. Gorringe, S.-F. Chin, P. Pharoah, J. M. Staines, C. Oliveira, P. A.W. Edwards, and C. Caldas Evidence that both genetic instability and selection contribute to the accumulation of chromosome alterations in cancer Carcinogenesis, May 1, 2005; 26(5): 923 - 930. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Oda, Y. Maehara, Y. Ikeda, E. Oki, A. Egashira, Y. Okamura, I. Takahashi, Y. Kakeji, Y. Sumiyoshi, K. Miyashita, et al. Two modes of microsatellite instability in human cancer: differential connection of defective DNA mismatch repair to dinucleotide repeat instability Nucleic Acids Res., March 18, 2005; 33(5): 1628 - 1636. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Perez, F. Cruz, and P. Presa Distribution Properties of Polymononucleotide Repeats in Molluscan Genomes J. Hered., January 1, 2005; 96(1): 40 - 51. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ishikawa, T. Fujita, Y. Suzuki, S. Okabe, Y. Yuasa, T. Iwai, and Y. Kawakami Tumor-specific Immunological Recognition of Frameshift-mutated Peptides in Colon Cancer with Microsatellite Instability Cancer Res., September 1, 2003; 63(17): 5564 - 5572. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Yoshino, S. Fukuyama, T. Kameyama, Y. Shikada, S. Oda, Y. Maehara, and K. Sugimachi Detection of Loss of Heterozygosity by High-Resolution Fluorescent System in Non-small Cell Lung Cancer: Association of Loss of Heterozygosity With Smoking and Tumor Progression Chest, February 1, 2003; 123(2): 545 - 550. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Raedle, J. Trojan, A. Brieger, N. Weber, D. Schafer, G. Plotz, E. Staib-Sebler, S. Kriener, M. Lorenz, and S. Zeuzem Bethesda Guidelines: Relation to Microsatellite Instability and MLH1 Promoter Methylation in Patients with Colorectal Cancer Ann Intern Med, October 16, 2001; 135(8_Part_1): 566 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Sobrido, C. R. Pereira, F. Barros, J. Forteza, A. Carracedo, and M. Lema Low frequency of replication errors in primary nervous system tumours J. Neurol. Neurosurg. Psychiatry, September 1, 2000; 69(3): 369 - 375. [Abstract] [Full Text] [PDF]
|
|