Nucleic Acids Research, Vol 25, Issue 17 3497-3502, Copyright © 1997 by Oxford University Press
NE Mollegaard, C Bailly, MJ Waring and PE Nielsen
Gel migration and uranyl photoprobing have been used to study the effects
of inosine and 2,6-diaminopurine (2,6-DAP) substitution on adenine-tract
(A-tract) induced DNA curvature. Using a 10mer repeated sequence including
five inosines we show by uranyl photoprobing that a narrow minor groove
varying in phase with the helix repeat is not the cause of DNA curvature.
Further, we have systematically studied by gel migration the effects on
A-tract induced curvature of either single or full substitution with
inosine and/or 2,6-DAP in a 5'-AAAAAGCCGC- 3'sequence. DNA curvature is
shown to increase when inosines are substituted for the guanosines in the
sequence between the A-tracts. By comparing the effects of each
monosubstitution it can be seen that when the G closest to the 3'-end of
the A-tract is substituted the effect on DNA curvature is much stronger
than when substitution is made at any other position. By contrast,
curvature is abolished when 2,6-DAP residues are substituted for all
adenines, and monosubstitution reveals that the effect of substituting a
single adenine is strongest at the 3'- end of the A-tract. These results
favor a model in which the curvature induced by an A-tract in DNA molecules
is primarily located at the junction with the 3'-end of the A-tract, and
this peculiar junction is created because the A-tract has a preference to
form a non-B-DNA structure which builds up from the 5'-end.
ARTICLES
Effects of diaminopurine and inosine substitutions on A-tract induced DNA curvature. Importance of the 3'-A-tract junction
Center for Biomolecular Recognition, Department of Medical Biochemistry and Genetics, The Panum Institute, University of Copenhagen, Blegdamsvej 3c, 2200 Copenhagen N, Denmark.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Lu and N. C. Stellwagen Monovalent Cation Binding by Curved DNA Molecules Containing Variable Numbers of A-Tracts Biophys. J., March 1, 2008; 94(5): 1719 - 1725. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Stellwagen, Y. Lu, and N. C. Stellwagen Curved DNA molecules migrate anomalously slowly in free solution Nucleic Acids Res., August 5, 2005; 33(14): 4425 - 4432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Lu, B. D. Weers, and N. C. Stellwagen Intrinsic Curvature in the VP1 Gene of SV40: Comparison of Solution and Gel Results Biophys. J., February 1, 2005; 88(2): 1191 - 1206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Mauro, D. Pawlowski, and G. B. Koudelka The Role of the Minor Groove Substituents in Indirect Readout of DNA Sequence by 434 Repressor J. Biol. Chem., April 4, 2003; 278(15): 12955 - 12960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Warmlander, J. E. Sponer, J. Sponer, and M. Leijon The Influence of the Thymine C5 Methyl Group on Spontaneous Base Pair Breathing in DNA J. Biol. Chem., August 2, 2002; 277(32): 28491 - 28497. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Langdon, C. Seago, R. N. Jones, H. Ougham, H. Thomas, J. W. Forster, and G. Jenkins De Novo Evolution of Satellite DNA on the Rye B Chromosome Genetics, February 1, 2000; 154(2): 869 - 884. [Abstract] [Full Text]
|
|