Nucleic Acids Research, Vol 25, Issue 18 3564-3569, Copyright © 1997 by Oxford University Press
WJ Ma, S Chung and H Furneaux
The Elav-like proteins are specific mRNA binding proteins which are
required for cellular differentiation. They contain three characteristic
RNP2/RNP1-type RNA binding motifs. Previously we have shown that the first
and second RNA binding domains bind to AU-rich elements in the 3'-UTR of
mRNA. In this paper we show that the Elav- like proteins exhibit poly(A)
binding activity. This activity is distinct from poly(A) binding activities
that have been previously described. The Elav-like proteins specifically
bind to long chain poly(A) tails. We have shown that the third RNA binding
domain encompasses this poly(A) binding activity. Using poly(A)-Sepharose
beads in a 'sandwich' assay we have shown that the Elav-like proteins can
bind simultaneously to the AU-rich element and to the poly(A) tail.
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The Elav-like proteins bind to AU-rich elements and to the poly(A) tail of mRNA
Program in Molecular Pharmacology and Therapeutics, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021 USA.
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