Nucleic Acids Research, Vol 25, Issue 18 3712-3717, Copyright © 1997 by Oxford University Press
H Ihn and M Trojanowska
Sp3 is an ubiquitously expressed transcription factor, closely related to
Sp1 but, unlike Sp1, it often functions as a transcriptional repressor. In
this study we investigated the role of Sp3 in regulating the transcription
of the human alpha2(I) collagen gene. We show that Sp1 and Sp3 specifically
bind to three of the previously characterized cis -elements in this
promoter, including two positive cis-elements between -303 and -271 and
-128 and -123, and a repressor site between - 164 and -159, but do not bind
to the fourth cis-element bound by CBF. Functional analyses of Sp3 and Sp1
in Drosophila cells indicate that each protein transactivates the human
alpha2(I) collagen promoter with equal potency and, when tested together,
have an additive effect on the promoter activity. Furthermore, in vitro
transcription assays demonstrate that both Sp1 and Sp3 are capable of
supporting transcription from the collagen promoter independently of each
other. However, when activities of both Sp1 and Sp3 are blocked with
specific antibodies, in vitro transcription from this promoter is almost
completely abolished. The results of this study demonstrate that Sp3 is as
potent an activator of the human alpha2(I) collagen promoter as is Sp1 and
that a transcriptional activity of the human alpha2(I) promoter is
dependent on both proteins.
ARTICLES
Sp3 is a transcriptional activator of the human alpha2(I) collagen gene
Department of Medicine, Division of Rheumatology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425-2229, USA.
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