Nucleic Acids Research, Vol 25, Issue 19 3795-3800, Copyright © 1997 by Oxford University Press
M Livingstone-Zatchej, A Meier, B Suter and F Thoma
Yeast uses nucleotide excision repair (NER) and photolyase
(photoreactivation) to repair cyclobutane pyrimidine dimers (CPDs)
generated by ultraviolet light. In active genes, NER preferentially repairs
the transcribed strand (TS). In contrast, we recently showed that
photolyase preferentially repairs the non-transcribed strands (NTS) of the
URA3 and HIS3 genes in minichromosomes. To test whether photoreactivation
depends on transcription, repair of CPDs was investigated in the
transcriptionally regulated GAL10 gene in a yeast strain deficient in NER
[AMY3 (rad1Delta)]. In the active gene (cells grown in galactose),
photoreactivation was fast in the NTS and slow in the TS demonstrating
preferential repair of the NTS. In the inactive gene (cells grown in
glucose), both strands were repaired at similar rates. This suggests that
RNA polymerases II blocked at CPDs inhibit accessibility of CPDs to
photolyase. In a strain in which both pathways are operational [W303-1a
(RAD1)], no strand bias was observed either in the active or inactive gene,
demonstrating that photoreactivation of the NTS compensates preferential
repair of the TS by NER. Moreover, repair of the NTS was more quickly in
the active gene than in the repressed gene indicating that transcription
dependent disruption of chromatin facilitates repair of an active gene.
ARTICLES
RNA polymerase II transcription inhibits DNA repair by photolyase in the transcribed strand of active yeast genes
Institut fur Zellbiologie, ETH-Zurich, Honggerberg, CH-8093 Zurich, Switzerland.
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