Nucleic Acids Research, Vol 25, Issue 19 3863-3867, Copyright © 1997 by Oxford University Press
A Di Polo, LE Lerner and DB Farber
During photoactivation retinal cGMP-phosphodiesterase (PDE) mediates signal
transduction in the photoreceptor outer segments. Mutations in the
beta-subunit gene of rod-specific PDE (beta-PDE) have been associated with
inherited retinal degeneration in a number of species, including human.
Here we have investigated the proximal upstream sequences that participate
in transcriptional activation of this gene. Transient transfections
demonstrated that the sequence from -72 to +53 bp contained sufficient
information to direct high levels of gene expression in cells of retinal
origin. Deletion or mutagenesis of an AP- 1 motif present in this region
caused 90-95% reduction in reporter gene expression. By gel mobility shift
assay we demonstrated specific interactions between putative nuclear
transcription factors and this AP- 1 element. These findings indicate that
the proximal AP-1 site in the human beta-PDE promoter is functionally
relevant and necessary for transcriptional activation of this gene.
ARTICLES
Transcriptional activation of the human rod cGMP-phosphodiesterase beta- subunit gene is mediated by an upstream AP-1 element
Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, CA 90095, USA.
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