Nucleic Acids Research, Vol 25, Issue 20 3974-3979, Copyright © 1997 by Oxford University Press
M Lombaerts, M Tijsterman, RA Verhage and J Brouwer
The recently cloned Saccharomyces cerevisiae MMS19 gene appears to be
involved in both nucleotide excision repair (NER) and transcription, which
is also the case for components of the NER/transcription complex TFIIH.
Unlike TFIIH however, the Mms19 protein does not affect NER in a highly
purified in vitro system. In order to investigate the role of Mms19 in NER,
we have analysed the repair capacity of the mms19 disruption mutant. We
find that a cell-free extract of this mutant is deficient for NER in vitro.
Since mms19 mutants are only moderately sensitive to irradiation with
ultraviolet (UV) light, it is possible that such mutants are specifically
deficient in one of the two modes of NER, i.e. transcription-coupled or
global genome repair. To investigate this possibility, we have analysed the
removal of cyclobutane- pyrimidine dimers (CPDs) at the nucleotide level in
an mms19 mutant. Repair of CPDs was not detectable for both transcribed and
non- transcribed sequences in this mutant, demonstrating a requirement for
Mms19 in both transcription-coupled and global genome repair. Our data,
combined with those obtained by others, suggest that Mms19 is required for
NER in yeast, although it seems likely that the protein plays an indirect
role in this process.
ARTICLES
Saccharomyces cerevisiae mms19 mutants are deficient in transcription- coupled and global nucleotide excision repair
Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands.
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