Nucleic Acids Research, Vol 25, Issue 20 3995-4003, Copyright © 1997 by Oxford University Press
MR Pelletier, EN Hatada, G Scholz and C Scheidereit
The expression of immunoglobulin (Ig) genes depends on tissue-specific
elements in the promoter and enhancer regions of light chain and heavy
chain genes. In contrast to the complex modular character of Ig enhancers,
the promoters appear to be simple, depending primarily on a conserved TATA
box and octamer elements. We have analyzed the role of proximal sequences
for Igkappa promoter function. Igkappa promoter transcription critically
depends on initiator-like sequences and on a downstream element located at
+24 to +39 relative to the start site. Replacement of these sequences
resulted in strong reduction of promoter activity. In vitro, these elements
were found to be more effective in extracts of lymphoid than of
non-lymphoid origin. Deletion of the downstream and initiation site regions
had a comparable effect on promoter activity to obliteration of the TATA
box or octamer element. The downstream sequence was bound by two nuclear
proteins, identical to the previously identified Ig-specific C5 and C6
complexes. Whereas C5 is found in HeLa cells and in lymphoid cells, C6 is
lymphoid specific. Thus, further specific sequences in addition to the
previously characterized elements, the octamer and the TATA box, are
required for efficient kappa promoter expression in B lymphocytes.
ARTICLES
Efficient transcription of an immunoglobulin kappa promoter requires specific sequence elements overlapping with and downstream of the transcriptional start site
Max-Delbruck-Center for Molecular Medicine MDC, Robert-Rossle-Str. 10, 13122 Berlin, Germany.
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