Nucleic Acids Research, Vol 25, Issue 20 4055-4060, Copyright © 1997 by Oxford University Press
AG Caoile and DB Stern
We have previously used a homologous in vitro transcription system to
define functional elements of the maize mitochondrial atpA promoter. These
elements comprise a central domain extending from -7 to +5, relative to the
transcription start site, and an upstream domain of 1-3 bp that is purine
rich and centered around positions -11 to -12. Within the central domain
lies an essential 5 bp core element. These elements are conserved in many
mitochondrial promoters, but their functionality has only been tested for
atpA. In this study we have introduced mutations into the corresponding
elements of two cox3 promoters and show that while the core element is
essential for cox3 promoter activity, upstream element mutations have
little or no effect. To define the minimal sequence required for in vitro
promoter activity a series of short cloned oligonucleotides corresponding
to the atpA promoter was used. While some activity was seen with a 14 bp
sequence, full activity required 26 bp, suggesting that elements other than
the core and upstream region can influence promoter strength. Another
series of clones showed that altered spacing between the upstream and core
elements of atpA had a significant effect on promoter activity. These
results further define important features of the plant mitochondrial
transcriptional machinery.
ARTICLES
A conserved core element is functionally important for maize mitochondrial promoter activity in vitro
Boyce Thompson Institute for Plant Research, Cornell University, Tower Road, Ithaca, NY 14853-1801, USA.
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